objectives: acinetobacter baumannii is a gram-negative, non-motile aerobic bacterium known as a nosocomial pathogen resisting often to broad range of antibiotics. the pathogen is a serious agent of mortality and morbidity in hospital particularly among immunocompromise patients. treatment and control of its infections is complicated owing to its high antibiotic resistance, survival in various e...

Radioimmunoprecipitation (RIP) analyses performed on 125I-surface-labeled Treponema pallidum cells using various immune sera revealed the presence of six major surface antigens (immunogens) with apparent molecular weights of 47 K, 36 K, 34 K, 32 K, 29 K, and 13 K. Among these, the 47 K surface antigen was most abundant. Radioimmunoprecipitation assays using 125I-labeled T. phagedenis biotype Re...

this study was carried out to determine the effects of immunogen as a prebiotic on growth-related parameters and gut micro-flora in r. kutum fry. a basal diet was formulated using common feed ingredients supplemented with immunogen at 0, 1, 1.5, and 2 g kg-1 leading to four experimental diets.  fish were randomly distributed in 500 l fiberglass tanks (1×1×0.5 m). the experiment lasted for eight ...

Significance HIV is a highly mutable pathogen that can mutate to evade vaccine-induced immune responses, thus negating the vaccine’s protective effects. If mutations responses incur fitness penalty for virus, other evolve partially compensate loss. Accounting such effects, we designed single long peptide immunogen comprised of parts proteins wherein would be difficult without penalties virus. T...

Dendritic cells (DC) can be divided into three subsets, Langerhans cells, myeloid DC (MDC), and lymphoid DC (LDC), based upon phenotypic and functional differences. We hypothesized that different DC subsets are associated with the development of protective vs nonprotective cell-mediated immune (CMI) responses against the fungal pathogen, Cryptococcus neoformans. To test this, mice were immunize...

Immunization with naked genes (DNA-immunization) is a perspective modern approach to prophylactic as well as therapeutic vaccination against pathogens, as well as cancer and allergy. A panel of DNA immunogens has been developed, some are already in the clinical trials. However, the immunogenicity of DNA vaccines, specifically of those applied to humans, needs a considerable improvement. There a...

This study was carried out to determine the effects of Immunogen as a prebiotic on growth-related parameters and gut micro-flora in R. kutum fry. A basal diet was formulated using common feed ingredients supplemented with Immunogen at 0, 1, 1.5, and 2 g kg-1 leading to four experimental diets.  Fish were randomly distributed in 500 L fiberglass tanks (1×1×0.5 m). The experiment lasted for eight ...

We have discovered an Aleuria Aurantia Lectin (AAL)-reactive immunoglobulin G (IgG) that naturally occurs in the circulation of rabbits and mice, following immune responses induced by various foreign antigens. AAL can specifically bind to fucose moieties on glycoproteins. However, most serum IgGs are poorly bound by AAL unless they are denatured or treated with glycosidase. In this study, using...

The prevention of bacterial infections via immunization presents particular challenges. While outer membrane extracts are often protective, they are difficult and expensive to isolate and standardize and thus are often impractical for development and implementation in vaccination programs. In contrast, individual proteins, which are easily adapted for use in subunit vaccines, tend to be poorly ...

The incidence of Clostridium difficile infection (CDI) and associated mortality have increased rapidly worldwide in recent years. Therefore, it is critical to develop new therapies for CDI. In this study, we generated a novel, potently neutralizing, tetravalent, and bispecific antibody composed of 2 heavy-chain-only VH (VHH) binding domains against both TcdA and TcdB (designated "ABA") that rev...