reply to the letter sent by prof. viroj wiwanitkit entitled “radiofrequency radiation and human ferritin”   authors of the article entitled “effects of radiofrequency radiation on human ferritin: an in-vitro enzymun assay” [1] would like to express their satisfactions, since the manuscript have been accessed and used as an element of reflection by a renowned researchers such as prof. wiwanitkit...

ferritin is a macromolecule and is responsible for the long term iron storage function in human serum and plasma. recent studies have highlighted the role of cell phone exposure on central nervous system, immune function and reproduction. the aim of this study was to investigate whether the human serum ferritin level could be interfered by the exposure to the 900 mhz gsm cell phones. fifty huma...

laboratory diagnosis of acute measles is usually achieved by serology assays for measle-specific igm antibody. for comparison of measle-specific igm antibody in saliva and serum, 95 paired blood and saliva samples were collected 1-14 days after the onset of rash. the specimens were tested for specific igm antibody by an igm antibody-capture enzyme immunoassay (eia). measles igm antibody was det...

We studied the EMIT (Enzyme Multiplied Immunoassay Technique, Syva) procedures for the assay of phenytoin and phenobarbital in serum, adapting them to the miniature Centrifugal Fast Analyzer. For different concentrations of drug, each rate of reaction decreased continuously with time, tending to converge on a single common value. The rate was most affected by the concentration of drug almost im...

An enzyme immunoassay technique based on the direct detection of Chlamydia trachomatis antigen in urethral or cervical swabs was used for the rapid diagnosis of chlamydial genital infection. Urethral and cervical samples from 140 patients were tested in parallel by enzyme immunoassay and cell culture using iodine staining. The direct test had a sensitivity of 92.5% and specificity of 97.2% when...

Published information on the major interactive variables of enzymic immunoassays for drugs, whether in urine or serum, is scanty. Using the Syva EMIT-dau Barbiturates assay as an example, we examined the following interactive variables with respect to test response: concentrations of enzyme, antibody, and drug; urine matrix; and time. The results suggest a process for optimizing this type of en...

We report a clinical evaluation of the enzyme immunoassay (EMIT) performed with the GEMSAEC centrifugal analyzer as compared to gas-liquid and liquid chromatography for anticonvulsant drugs and theophylline, respectively. A good correlation was obtained for all drugs, although some difficulties were experienced with one lot of reagent for ethosuximide. The analyzer has an economic advantage if ...

We assayed serum gentamicin and tobramycin specimens by the enzyme multiplied immunoassay technique (Syva EMIT) and the fluorescence polarization immunoassay (Abbott TDx). When interassay and intraassay control samples were evaluated, both methods gave an overall coefficient of variation of less than +/- 10%. Using patient serum samples, we obtained excellent correlation with both methods in th...

Using the enzyme immunoassay technique (EMIT), we determined the time course of urinary excretion of benzoylecgonine in 16 surgical patients and three volunteers who received intranasal cocaine. After doses varying from 13 to 130 mg, the test for benzoylecgonine was positive in 1 to 4 h, peaked at about 10 to 12 h, remained positive for 18 to 27 h, and became negative after about 17 h. This inf...

We describe specific EMIT homogeneous enzyme immunoassays for amitriptyline, nortriptyline, imipramine, and desipramine in patients' serum samples. Before analysis, an easily performed extraction step involving the use of 500 microL of sample and a 1-mL disposable column eliminates cross-reacting polar metabolites. The range of the standard curve for the first three drugs is 25 to 250 microgram...