abstract: potato virus y a type species of the genus potyvirus infects several crops in the family solanaceae. pvy isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. the result of elisa, immunoblot electrophoresis and immuno capture reverse transcription polymerase chain reaction   (ic-rt-pcr) indicated that pepper isol...

The study recorded for the first time in Egypt presence of tomato spotted wilt virus (TSWV) on cucurbits, which was confirmed using several serological tests, like double antibody sandwich ELISA (DAS-ELISA); Dot-blot assay and Immuno capture polymerase chain reaction (IC-PCR).  By Dot blot immunoassay test against TSWV antisera, ability thrips species Thrips tabaci (Lind), palmi (Karny), Frankl...

In the Western blot, HIV-1 or HIV-2 proteins are denatured, separated by electrophoresis in function of their molecular weight and transferred on a nitrocellulose strip. The presence of antibodies against one or several of these proteins is revealed by an immuno-enzymatic reaction producing a colored reaction. The result is interpreted with well-defined criteria. Incomplete profiles can be obse...

Background: The production of recombinant proteins in Escherichia coli is one of the most valuable achievements in biotechnology, with many therapeutic and diagnostic applications; however, the aggregation and misfolding of proteins that result in the formation of insoluble inclusion bodies is a disruptive factor in this process. Various solubilization and refolding methods can be used to impro...

Objective(s):Human Cytomegalovirus (HCMV) remains a major morbidity and mortality cause in immuno suppressed patients. Therefore, significant effort has been made towards the development of a vaccine. In this study, the expression of the pp65 and gB fusion peptides and Fc domain of mouse IgG2a as a novel delivery system for selective uptake of antigens by antigen-presenting cells (APCs) in Pich...

A vegetative storage protein (VSP) with trypsin inhibitor activity in a deciduous tree, Sapindus mukorassi, was characterized by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western-blot, immuno-histochemical localization, light- and electro-microscopy, together with analysis of proteinase inhibitor activity of the purified VSP in vitro. There were two proteins with molec...

objective(s):human cytomegalovirus (hcmv) remains a major morbidity and mortality cause in immuno suppressed patients. therefore, significant effort has been made towards the development of a vaccine. in this study, the expression of the pp65 and gb fusion peptides and fc domain of mouse igg2a as a novel delivery system for selective uptake of antigens by antigen-presenting cells (apcs) in pich...