An overview of the use of immobilized enzymes in flow-injection analysis (FIA) is presented. The joint use of FIA and immobilized enzymes means that analytical procedures are easily automated, analytical costs are reduced and methods are faster. The future possibilities for this combination are discussed.

We introduce a two-dimensional lattice model of immobilization and stabilization of proteinlike polymers using grafted polymers. The protein is designed to have a specific bulk conformation reproducing a catalytic cleft of natural enzymes. Our model predicts a first order denaturing adsorption transition of free proteins. On the other hand, for an immobilized protein we observe a more gradual d...

A nanostructured biocatalytic interface reversibly gating electrochemical reactions upon chemical signals processed by immobilized enzymes was architectured. The chemical signals were converted to local interfacial pH changes causing restructuring of the stimuli-responsive polymer and switching ON-OFF the electrochemical reaction.

www.MaterialsViews.com C O M M U Nanoporous Scaffold with Immobilized Enzymes during Flow-Induced Gelation for Sensitive H 2 O 2 Biosensing N IC A By Donglai Lu , Joshua Cardiel , Guozhong Cao , and Amy Q. Shen * IO N Biosensors play indispensible roles in disease diagnosis, drug screening, and forensic applications, while nanoporous scaffolds hold an enormous potential in improving the perform...

Improvement of properties by immobilization http://www.scitopics.com/IMPROVEMENT_OF_ ENZYME_PROPERTIES_VIA_IMMOBILIZATION_ TECHNIQUES.html This webpage focuses on the means by which enzyme activity can be stabilized or improved in an immobilized state compared with the same enzyme free in aqueous solution. Immobilized enzymes http://www.rpi.edu/dept/chem-eng/Biotech-Environ/viet/ nelly.html Thi...

Background: Immobilization is an approach in industry to improve stability and reusability of urease. The efficiency of this technique depends on the type of membrane and the method of stabilization. Methods: The PEI-modified egg shell membrane was used to immobilize urease by absorption and glutaraldehyde cross-linking methods. The membranes were characterized by Fourier-transform infrared sp...

The advantages of immobilized over soluble enzymes arise from their enhanced stability and ease of separation from the reaction media, leading to significant savings in enzyme consumption. Immobilization methods range from binding to prefabricated carrier materials to packaging in enzyme crystals or powders. During their use, mass-transfer effects can produce substrate or pH gradients, which re...