bakground: multiple sclerosis (ms) is a cd4+ t cell-mediated autoimmune disease affecting the central nervous system (cns). it was previously believed that th1 cells were pathogenic t cells in experimental autoimmune encephalomyelitis (eae). however, the functional role of th1 cells in eae has been reconsidered upon the discovery of il-17- producing t cells which are consider as dominant effect...

antisense oligodeoxynucleotides (oligos) are the tools that bind to complemen¬tary sequence of targeted mrna and block specifically protein translation. in the present study, a novel 20 mer oligo as an antisense for human il-13 is introduced. this oligo is designed according to the il-13 mrna coding region and synthesized in two hplc purified and fitc conjugated forms. fluorescence oligo cell u...

Interleukin-13 (IL-13) belongs to the IL-4 gene family. Like IL-4, IL-13 induces IL-1 receptor antagonist (IL-1Ra) synthesis with no effect on IL-1beta synthesis. We investigated whether IL-13 induces IL-1Ra synthesis via a pathway similar to IL-4. In human peripheral blood mononuclear cells, IL-13 (1 to 100 ng/mL alone induced IL-1Ra synthesis in a dose-dependent manner. A single amino acid mu...

BACKGROUND Our laboratory has demonstrated previously that human tonsillar B lymphocytes express IL-13 mRNA OBJECTIVE: We sought to investigate IL-13 production by human B cells and the association between B cell-derived IL-13 and IgE secretion. METHODS Human B lymphocytes were isolated from tonsils and purified by means of rosetting with sheep RBCs or positive or negative selection with magn...

The allergen-induced late nasal response (LNR) is associated with high expression of interleukin-4 (IL-4) and IL-5 messenger RNA (mRNA) in the nasal mucosa, suggesting a role for Th2-type cytokines in the development of the LNR. Moreover, topical corticosteroid-mediated inhibition of the LNR is accompanied by inhibition of IL-4, but not IL-5, mRNA expression, IL-13 shares a number of functions ...

Background: IL-4 is a cytokine that induces differentiation of naive helper T cells into Th2 cells. Once activated by IL-4, Th2 cells subsequently produce additional IL-4. Objective: To examine the effect of IL-4 on IL-17 production and its effect in Collagen- Induced Arthritis (CIA) mice. Method: In this study, a chicken collagen-II-induced experimental arthritis (CIA) model was used in DBA/1 ...

Zhou, XiuXia, John B. Trudeau, Kathryn J. Schoonover, Jessica I. Lundin, Steve M. Barnes, Meghan J. Cundall, and Sally E. Wenzel. Interleukin-13 augments transforming growth factor1induced tissue inhibitor of metalloproteinase-1 expression in primary human airway fibroblasts. Am J Physiol Cell Physiol 288: C435– C442, 2005. First published September 29, 2004; doi:10.1152/ ajpcell.00035.2004.—Ti...

Tissue inhibitor of metalloproteinase (TIMP)-1 is a potent inhibitor of activated matrix metalloproteinases (MMPs) such as gelatinases and collagenases. TIMP-1 is induced by transforming growth factor-beta1 (TGF-beta1), but details regarding signaling pathways remain unclear. T-helper-2 cytokines also have profibrotic properties and can interact with TGF-beta. In the present study, we examined ...

AIM To identify and characterize the subunit structure of interleukin-13 receptor (IL-13R) in human medulloblastoma cell lines and target them with a chimeric fusion protein consisting of interleukin-13 and Pseudomonas exotoxin (termed as IL-13 cytotoxin). METHODS Five human medulloblastoma cell lines were examined for the expression of IL-13R subunits at the mRNA and protein levels by revers...