نتایج جستجو برای: igy technology

تعداد نتایج: 468457  

Journal: :ALTEX 1996
Rüdiger Schade Andreas Hlinak

Immunization of chickens and extraction of antibodies from egg yolk belongs to the alternative methods since the animals suffering is reduced by non-invasive antibody-sampling. Also, the number of animals needed to produce a certain amount of antibody can be reduced since chickens produce a significant higher antibody quantity than rabbits. Despite its several advantages this technology (IgY-te...

2006
R. SCHADE

The IgY-technology stand for the immunisation of chickens and the extraction of specific IgY antibodies (Ab) from egg yolk. Due to the non-invasive Ab isolation IgY-technology is internationally accepted as so-called alternative method. Despite of the aspect of animal welfare Ab production in chicken offers also outstanding economical advantages. i) purchase of chickens is much more cheep than ...

Journal: :modares journal of medical sciences: pathobiology 2008
hossein basiri seyed latif mousavi gargari iraj rasoli ali hatef salmanian kazem parivar

objective: helicobacter pylori is a spiral, microaerophilic gram negative bacterium, that multiplies and causes infection in human gastric mucosal layer. h.pylori infection, followed by destruction of gastric epithelial tissue, leads to gastric chronic inflammation, which can cause gastric and peptic ulcers. new approaches have focused on using specific treatments, such as immunotherapy, to era...

2017
Kamrul Hasan Khan Arisa Himeno Shouhei Kosugi Yosuke Nakashima Abdur Rafique Ayana Imamura Takaaki Hatanaka Dai-Ichiro Kato Yuji Ito

Chicken egg yolk immunoglobulin (IgY) is a functional substitute for mammalian IgG for antigen detection. Traditional IgY purification methods involve multi-step procedures resulting in low purity and recovery of IgY. In this study, we developed a simple IgY purification system using IgY-specific peptides identified by T7 phage display technology. From disulfide-constrained random peptide libra...

Journal: :Poultry science 2007
C C Constantinoiu J B Molloy W K Jorgensen G T Coleman

Immunoglobulin Y is different from most of the other immunoglobulins because it does not bind protein A or protein G. Thiophilic gel chromatography has been successfully used to purify IgY from chicken egg yolk, but the technology has not previously been used to purify IgY from serum. In this research note, we describe the optimization of T-gel chromatography for purification of IgY from serum....

Journal: :Separation and Purification Technology 2022

Although their high potential as alternative biopharmaceuticals, less than 2% of the total polyclonal antibodies produced worldwide correspond to immunoglobulin Y (IgY) due difficulties in isolating them from egg yolk (complex biological matrix). In this work, water-soluble proteins fraction (WSPF) was first obtained and present identified by one-dimensional gel electrophoresis (SDS-PAGE) label...

2008
H. M. Elsheikha Abdel Aziz

The focus of this report was to assess the role of passive immunization using immunoglobulins derived from chicken egg yolk in protection against M. gallisepticum infection in chickens. Immunoglobulins were produced by egg yolk technology. ELISA was used to evaluate their immunoreactivity and western immunoblotting demonstrated that chicken egg yolk immunoglobulins Y (IgY) were capable of recog...

2013
Dary Luz Mendoza Gloria Garavito Luis Alejandro Barrera Angela Espejo Elkin Navarro Eduardo Egea

Background One of the main strategies to reduce the expression of allergic symptoms in atopic patients sensitized to house dust mites is the use of detection system for monitoring and reduce environmental allergens in indoor spaces. In this context the IgY technology represent a valuable tool. The specific aims of this study was to standardize a in house method of indirect ELISA assay for detec...

ژورنال: Medical Laboratory Journal 2016
Hashemzadeh Farhang , Hossein, Shahbazi , Parisa,

ABSTRACT          Background and Objectives: Cryptosporidium parvum is a coccidian protozoan that causes diarrhea in immunocompromised humans and newborn animals. The severity of the disease depends on the immunological status of the affected. Cryptosporiosis can have lethal effects on immunocompromised individuals such as AIDS patients. About 10% of...

Journal: :iranian journal of basic medical sciences 0
khadijeh nasiri department of animal science, faculty of agriculture, ferdowsi university of mashhad, mashhad , iran saeed zibaee razi vaccine and serum research institute, agricultural research, education and extension organization (areeo), mashhad, iran mohammadreza nassiri department of animal science and institute of biotechnology, ferdowsi university of mashhad, mashhad, iran mojtaba tahmoorespur department of animal science, faculty of agriculture, ferdowsi university of mashhad, mashhad , iran alireza haghparast department of veterinary medicine, faculty of veterinary medicine, ferdowsi university of mashhad, mashhad, iran

objective(s): enterotoxigenic escherichia coli (etec) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. igy technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. the purpose of this study was to produce specific antibody by egg yolk antibody (igy...

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