The manipulation of redox potential in secretory pathway by thiol reducing agents can be a strategy to improve the production levels of disulfide-bonded proteins including recombinant antibodies. Here we have studied the influence of cysteamine on viability and the production level of IgG4 in Sp2.0 cells. For this purpose, the recombinant Sp2.0 cells producing an anti CD33 IgG4, were subjected ...

The manipulation of redox potential in secretory pathway by thiol reducing agents can be a strategy to improve the production levels of disulfide-bonded proteins including recombinant antibodies. Here we have studied the influence of cysteamine on viability and the production level of IgG4 in Sp2.0 cells. For this purpose, the recombinant Sp2.0 cells producing an anti CD33 IgG4, were subjected ...

Patients with IgG4-related disease (IgG4-RD) are diagnosed in Japan by comprehensive or organ-specific diagnostic criteria. To date, criteria have been established for several organs, but not the thyroid. We attempted to establish thyroid (IgG4-RTD) based on IgG4-RD research The Research Program Intractable Diseases from Ministry of Health, Labour and Welfare Japan. These publicly reported memb...

Serum IgG subclass levels were measured using an indirect immunoenzymatic assay (ELISA) with monoclonal antibodies in 16 children with asthma and 13 children with atopy who had mostly recurrent infections. Seven of the asthmatic children had marked low or low normal levels of IgG4, six had marked low or low normal levels of IgG 3, two had marked low normal levels of both IgG2, and IgG 3 and...

â  background : primary sclerosing cholangitis (psc) is a chronic cholestatic disease that results in segmental stenosis of the bile ducts, cholestasis and fibrosis. during the previous two decades, patients with steroid responsive psc have been presented in a few case reports and studies. this study aims to evaluate and compare igg4 levels in patients with ulcerative colitis (uc), with and wit...

IgG4-related disease (IgG4-RD) is a potentially multiorgan disorder. In this study, clinical and serological features from 132 IgG4-RD patients were compared about organ correlations. Underlying pathologies comprised autoimmune pancreatitis (AIP) in 85 cases, IgG4-related sclerosing cholangitis (IgG4-SC) in 12, IgG4-related sialadenitis (IgG4-SIA) in 56, IgG4-related dacryoadenitis (IgG4-DAC) i...

•Elevated IgG4 levels and IgG4/IgG ratios are not specific for IgG4-RD. •IgG4-RD causes most elevated high above the upper limit of normal. •Even at very serum levels, 25% patients did have

The Fc fragment of IgG4 can interact with the Fc fragment of another IgG molecule. This interaction is a confounding factor when measuring IgG4 rheumatoid factor levels. Recently, we demonstrated that half-molecules of IgG4 can exchange to form a bispecific Ab. We expected these two phenomena to be related and investigated the physicochemical aspects of IgG4 Fc-Fc interactions. We found that Ig...

In our case, we present a case of an 80-year-old male who was referred to the gastroenterologist for evaluation suspicious mass. CT imaging at time had shown intrahepatic and extrahepatic biliary dilations, patient thought have pancreatic or common bile duct A mass biopsy showed no malignancy, further warranted. The found elevated IgG4 levels diagnosed with IgG4-sclerosing cholangitis (IgG4-SC)...