background and aims: toxoplasmosis is a parasitic disease which may cause some laboratory symptoms in infected individuals. one of the main ways of transmition this organism is placenta to fetus pathway. if this transmission occurs in the 3th month of pregnancy, the abortion, central nerve system and ocular disorder will happen. because of this issue, the precise technique for detection of toxo...

different igg subclass profiles are produced in response to different antigenic stimuli in a variety of diseases. igg subclass levels may reflect disease severity. quantification of igg subclasses depends on the availability of specific monoclonal antibodies (mabs). in the present study seven hybridoma clones producing mabs reactive with multiple subclasses of human igg were established. spleno...

background:infection of mothers with rubella virusduring pregnancy can be serious; if the mother is infected within the first 20 weeks of pregnancy she is likely to have miscarriage, stillbirth, or baby with congenital rubella syndrome. this study was carried out to define rubella virusseroprevalence in pregnancy in osogbo, nigeria. methods:this study is a cross-sectional sero-survey of rubella...

objective: to evaluate the importance of cytokine type in embryo implantation in uterus specified and activated macrophages interfere the tube movements and embryo retention in uterine tubes by smooth muscle relaxation and disrupting ciliary function. therefore, increased risk of infection with hp during pregnancy, we investigated relation between helicobacter pylori (hp) infection and prevalen...

Background: Decay of vaccine–induced antibody titres without boosting of the wild measles virus has been well documented. Revaccination against measles has reduced the prevalence of the disease worldwide. Revaccination may cause IgE induced anaphylaxis. Objective: To study measles IgG antibody in revaccinated populations and its relation to IgE induced hypersensitivity. Methods: Blood samples w...

Antibodies are essential tools of biomedical and biochemical researches. Polyclonal antibodies are produced against different epitopes of antigens. Purified F(ab')2 can be used for animal’s immunization to produce polyclonal antibodies. Human immunoglobulin G (IgG) was purified by ion exchange chromatography method. In all stages verification method of the purified antibodies was sod...

background: the affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as elisa. affinity of most igg specific mabs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...

background: celiac disease is a common autoimmune disorder that is diagnosed based on clinical case identification, serological screening, and duodenal histology. however, the existence of mild clinical forms, such as seronegative cases with patchy atrophy and potential celiac disease, can make it difficult to determine a definitive diagnosis. the seronegative patients with celiac disease can i...

recurrent abortion is a critical problem in which many factors play a crucial role such as anticordiolipin antibody and antiphospholipid antibody. this study was conducted to evaluate the frequency of anticardiolipin antibody and antiphospholipid antibody in pregnancy failures in women with the history of recurrent pregnancy loss. in 154 women with the history of two or more recurrent pregnancy...