A DNA probe derived from the subunit gene of a cloned 987P determinant was used to characterize the locations of the 987P genes in several Escherichia coli strains. We examined E. coli 987, a nonpiliated mutant of strain 987 (I36) that does not express 987P in vitro, and a variant of I36 that expressed 987P following growth in vivo. We found that plasmid and chromosomal copies of the 987P subun...

The amino acid at position 36 of the HIV-1 protease differs among various viral subtypes, in that methionine is usually found in subtype B viruses but isoleucine is common in other subtypes. This polymorphism is associated with higher rates of treatment failure involving protease inhibitors (PIs) in non-subtype B-infected patients. To investigate this, we generated genetically homogeneous wild-...

I36 1 1 Chao, H. H.-J., Waheed, A., Polhmann, R., Hille, A. and von Figura, K. (1990) EMBO J. 9, 3507-3513 12 Ma, Z., Grubb, J. H. and Sly, W. S. (1991) J. Biol. Chem. 266, 10589-10595 13 Johnson, K. F. and Kornfeld, S. (1992) J. Biol. Chem. 267, 17110-17113 14 Ludwig, T., Ruther, U., Metzger, R., Copeland, N. G., Jenkins, N. A., Lobel, P. and Hoflack, B. (1992) J. Biol. Chem. 267, 1221 1-12219...

The b 1 protein, produced in leaves of Nicotiana tabacum cv. Xanthi-nc following infection with tobacco mosaic virus, has been purified to homogeneity by a procedure which involves gel chromatography and absorption on to DEAEcellulose. One gel chromatography step was sufficient when the procedure was applied to leaf extracts made in an acid buffer, whereas two were necessary with extracts made ...

I I36 extract of the PM fraction was dominated by the strong actin band, while the corresponding fraction from ER had a particularly strong band at about 50 kDa. This band was also present in the NP-40 extract but was not observed in the 130 mwKCI extract. One band at about 60 kDa appeared exclusively in the Triton X-lOO/DOC fraction of ER. The results show that the three extracts from the PM-,...

PROD id="i32" type Definition id="i0" MUTIND uri="cic:Coq/init/Datatypes/nat.ind" notype="0" id="i33" source apply xref="i34" apply xref="i37" times definitionURL= "cic/Coq/Init/Peano/mult.con" xref="i38" 0 definitionURL= "cic:/Coq/Init/Datatypes/nat.ind" cn xref="i41" 0 definitionURL= xref="i40" "cic:/Coq/Init/Datatypes/nat.ind" cn eq xref="i35" "cic:/Coq/Init/Logic/Equality/eq.ind" definition...

Rational mutation of proteins based on their structural and dynamic characteristics is a useful strategy for amplifying specific fluctuations in proteins. Here, we show the effects of mutation on the conformational fluctuations and thermodynamic stability of ubiquitin. In particular, we focus on the salt bridge between K11 and E34 and the hydrogen bond between I36 and Q41, which are predicted t...