The electrochemical oxidation of iodide ion in the presence of 4-hydroxycoumarin (1) was studied using cyclic voltammetry and controlled-potential coulometry. The result indicates that the resulting iodine takes part in a halogenation reaction and reacts with 4-hydroxycoumarin (1). According to the obtained results, a new and simple coulometric titration method with potentiometric end-point det...

Reactions of 4-hydroxycoumarin 1 with heterocyclic aldehydes 2-4 led to bis-4-hydroxycoumarin derivatives 5-7 under microwave irradiation as well the classical heating. The subsequent reactions products are described. 4,4’-Epoxydicoumarins 8, 9 were prepared by reaction in acetic acid / p-toluenesulfonic medium. Compound 10 was 5 anhydride presence sodium acetate. Dioxocine-1,15-dione 11 6 dich...

Methoxsalen (8-methoxypsoralen) is an effective and selective mechanism-based inhibitor of human hepatic cytochrome P-450 (CYP)2A6 in vitro, and may have utility as a clinical probe for CYP2A6-catalyzed xenobiotic metabolism in humans in vivo. This investigation explored single-dose oral methoxsalen effects on human CYP2A6 activity in vivo, assessed by coumarin 7-hydroxylation. Eleven volunteer...

The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. To enable minimally invasive studies of proteins in their native context, it is desirable to tag proteins with small, bright reporter groups. Recently, our lab described PRIME technology (for PRobe Incorporation Mediated by Enzymes) for such tagging [1-3]. An engineered variant...

The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. To enable minimally invasive studies of proteins in their native context, it is desirable to tag proteins with small, bright reporter groups. Recently, our lab described PRIME technology (for PRobe Incorporation Mediated by Enzymes) for such tagging [1-3]. An engineered variant...

Abstract The preparation 0f azo dyes is the subject of this research. 4-hydroxycoumarin with each ofo-amino benzoic acid (3-[o-Amino acidazo]-4-hydroxycoumarin]) (B 1 ) and Sulfa guanidine (3-[Sulfa azo]-4-hydroxycoumarin) 2 were syntheses. Azo compounds characterized by I.R, CHN, H-NMR Elctronic Mass spectroscopy. At various pH levels, electronic spectra these investigated in terms acid-base p...

The in vitro biotransformation of 7-alkoxycoumarin by rat liver microsomes was studied to develop a simple and accurate assay procedure for 7-alkoxycoumarin O-dealkylase. 7-Alkoxycoumarin was converted to the O-dealkylated metabolite, 7-hydroxycoumarin, by aerobic incubation of the parent compound with microsomes and NADPH, but the decreased amount of 7-alkoxycoumarin in the reaction mixture wa...

Warfarin is often used as a site-specific probe for examining the binding of drugs and other solutes to Sudlow site I of human serum albumin (HSA). However, warfarin has strong binding to HSA and the two chiral forms of warfarin have slightly different binding affinities for this protein. Warfarin also undergoes a slow change in structure when present in common buffers used for binding studies....

A concise and efficient one-pot synthesis of 3-functionalized 4-hydroxycoumarin derivatives via a three-component domino reaction of 4-hydroxycoumarin, phenylglyoxal and 3-arylaminocyclopent-2-enone or 4-arylaminofuran-2(5H)-one under catalyst-free and microwave irradiation conditions is described. This synthesis involves a group-assisted purification process, which avoids traditional recrystal...

The hydrochloride of the racemic amino acid (7-hydroxycoumarin-4-yl)ethylglycine, a versatile fluorescent probe in proteins, has been synthesized in five steps from commercially available (7-hydroxycoumarin-4-yl)acetic acid. The key step involves the alkylation of a glycine-enolate equivalent.