Semen obtained from four Beluga males (Huso huso) was cryopreserved using extender; Tris-sucroseKCl (30mM Tris, 23.4mM sucrose, 0.25mM KCl, PH 8.0) supplemented with MeOH at concentration of 5%, 10% and 20%. Semen was diluted, respectively, with ratios of 1:0.5, 1:1 and 1:2 with extender and frozen in liquid nitrogen vapor.Frozen sperms after 30 and 60 days were excluded from freezing. Experime...