the applicability of hollow fiber-based liquid phase microextraction (hf-lpme) was evaluated for the extraction and preconcentration of loratadine prior to determination by hplc. in order to obtain high extraction recovery, the parameters affecting the hf-lpme including type of organic phase, ph of the source and receiving phases, stirring rate, extraction time and salt addition were studied an...

background: there are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. there are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

background and aims: a selective and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been established for determination of methotrexate in human plasma. methods: methotrexate was extracted from plasma with acetonitrile. the mobile phase consisted of acetonitrile-water-formic acid 74: 25: 1(v/v). twenty µl of sample was chromatographically an...

a simple, reproducible and rapid gas chromatographic method for precise determination of valproic acid (vpa) in human plasma has been developed. total time for sample preparation and gc analysis is less than 45 min. after plasma protein precipitation, vpa was extracted into chloroform with suitable recovery. by using stabilwax®-da capillary gc column, a symmetrical gas chromatographic peak was ...

Background: Due to multiple roles of albumin in the body, injection of its medicinal product as one of the therapeutic or management strategies under conditions such as severe bleeding, burns, liver failure, and neonatal hemolytic diseases is on the physicians' agenda. Considering that albumin is the most abundant plasma protein, designing an appropriate method to purify it is highly important....

in previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. the aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry to assay mebudipine in the human plasma. separation was achieved on a zorbax...

Background and aims: A selective and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been established for determination of methotrexate in human plasma. Methods: Methotrexate was extracted from plasma with acetonitrile. The mobile phase consisted of acetonitrile-water-formic acid 74: 25: 1(v/v). Twenty...

        A simple, reproducible and rapid gas chromatographic method for precise determination of valproic acid (VPA) in human plasma has been developed. Total time for sample preparation and GC analysis is less than 45 min. After plasma protein precipitation, VPA was extracted into chloroform with suitable recovery. By using Stabilwax®<span styl...

For the first time, a novel and efficient ionic liquid-based ultrasound-assisted in-situ solvent formation microextraction (IL-UA-ISFME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) has been successfully developed for the determination of duloxetine (DLX) in human plasma. Herein, an environmentally-friendly hydrophobic ionic liquid (1-butyl-3-methylimidaz...

cefixime is essential member of orally energetic third generation cephalosporin and has tremendous activity aligned with many pathogens. the virtual bioavailability of a newly industrial dispersible tablet as compared with a recognized identified formulation. a simple and available reversed-phase hplc method with uv detection has been urbanized and validate for cefixime evaluate in human plasma ...