introduction: osteoblasts and periodontal ligament cells are major cells for wound healing after root end resection. the interaction of osteoblasts with filling materials could play a critical role in healing of surgical lesion. adhesion and spreading of cells on material surface are the initial phase for cellular function. the purpose of the present study was the evaluation of morphology and a...

background: characteristics of differentiated osteoblasts from adipose derived stem cells (adscs) in compared with isolated osteoblasts from normal bone such as calvaria are unknown. the aim of this study was determination and comparison of phenotypic characterization between differentiated osteoblasts from stem cells and calvaria osteoblasts in vitro . methods: in this study, mesenchymal stem ...

Objective(s): Previous study has indicated that triiodothyronine (T3) facilitated cartilage degeneration in osteoarthritis (OA). This study aimed to investigate the effects of T3 on angiogenesis-related factor expression in human osteoblasts of OA subchondral bone.Materials and Methods: The subchondral bone specimens were obtained from O...

Staphylococcus aureus is the bacterial pathogen which is responsible for approximately 80% of all cases of human osteomyelitis. It can invade and remain within osteoblasts. The fate of intracellular Staph. aureus after the death of the osteoblast has not been documented. We exposed human osteoblasts to Staph. aureus. After infection, the osteoblasts were either lysed with Triton X-100 or trypsi...

introduction: the materials used for root-end filling and perforation repair are in direct contact with live tissues e.g. bone and connective tissue; their effects however, are uncertain. the aim of this ex vivo study was to evaluate the osteoblastic secretory activity adjacent to gray and white mineral trioxide aggregate (mta) and intermediate restorative material (irm). materials & methods: t...

objectives: osteoblasts are the most important cells in the osseointegration process. despite years of study on dental implants, limited studies have discussed the effect of saliva on the adhesion process of osteoblasts to implant surfaces. the aim of this in vitro study was to evaluate the effect of saliva on morphology and differentiation of osteoblasts attached to implant surfaces. materials...

Activation of mTOR complex 1 (mTORC1) could protect human osteoblasts from dexamethasone. Tuberous sclerosis complex 1 (TSC1) is mTORC1 upstream inhibitory protein. We demonstrate here that microRNA-19a ("miR-19a", -3p) targets the 3' untranslated regions of TSC1 mRNA. Expression of miR-19a downregulated TSC1 in OB-6 osteoblastic cells and primary human osteoblasts. miR-19a activated mTORC1 and...

We cultured human osteoblasts from trabecular bone explants and confirmed their phenotype by alkaline phosphatase assay, increased cyclic adenosine monophosphate production in response to prostaglandin E2 and radiographic micro-analysis of nodules of calcification. The osteoblasts were seeded on to demineralised human bone fragments and examined at ten-day intervals over a 50-day period by scan...