introduction: the materials used for root-end filling and perforation repair are in direct contact with live tissues e.g. bone and connective tissue; their effects however, are uncertain. the aim of this ex vivo study was to evaluate the osteoblastic secretory activity adjacent to gray and white mineral trioxide aggregate (mta) and intermediate restorative material (irm). materials & methods: t...

background: clinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. however, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. this study was performed to investigate whether...

Background: Clinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. Flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. However, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. This study was performed to investigate whether...

BACKGROUND/AIMS The antigen phenotype of human osteoblast-like cells suggests that they are related to other cellular populations and may also have immunologic functions in common. METHODS Flow cytometry and transmission electron microscopy were used to show the phagocytotic activity of osteoblast-like cells in culture. The allogeneic stimulation of T cells by human osteoblast-like cells was ...

introduction: the purpose of this study was to investigate the effect of 6w power carbon dioxide laser (co2) on the biologic compatibility of the sandblasting with large grit and acid etching (sla) titanium discs through studying of the sarcoma osteogenic (saos-2) human osteoblast-like cells viability. methods: sterilized titanium discs were used together with saos-2 human osteoblast-like cells...

Introduction: Biomechanical stimulation of cultured human osteoblast-like cells, which is based on controlled mechanical vibration, has been previously indicated, but the exact parameters that are effective for cells' proliferation enhancement still elusive due to lack direct data recordings from stimulated cells in culture. Therefore, we developed a low friction tunable system enables recordin...

The formation of cranial bone requires the differentiation of osteoblasts from undifferentiated mesenchymal cells. The balance between osteoblast recruitment, proliferation, differentiation and apoptosis in sutures between cranial bones is essential for calvarial bone formation. The mechanisms that control human osteoblasts during normal calvarial bone formation and premature suture ossificatio...

The most biologically active metabolite 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) has well known direct effects on osteoblast growth and differentiation in vitro. The precursor 25-hydroxyvitamin D3 (25(OH)D3) can affect osteoblast function via conversion to 1,25(OH)2D3, however, it is largely unknown whether 25(OH)D3 can affect primary osteoblast function on its own. Furthermore, 25(OH)D3 is not o...

Osteoblasts (OBs) and osteoclasts (OCs) are 2 major groups of bone cells. Their cell-to-cell interactions are important to ensure the continuity of the bone-remodeling process. Therefore, the present study was carried out to optimize an OB/OC co-culture system utilizing the human OB cell line hFOB 1.19 and OCs extracted from peripheral blood mononuclear cells (PBMNCs). It was a 2-step procedure...