Urinary protein obtained from a patient with a highly malignant brain tumor (astrocytoma, grade IV) was adsorbed to trimethylsilyl controlled-pore glass beads and selectively eluted with acetonitrile to yield a high molecular weight (HMW) human transforming growth factor (hTGF). This HMW hTGF promoted clonogenic cell growth in soft agar and competed for membrane receptors with mouse epidermal g...

epidermal growth factor (egf), a growth factor involved in cell growth and differentiation, is a small polypeptide with molecular weight of approximately 6 kda known to be present in a number of different mammalian species. experimental studies in animals and humans have demonstrated that the topical application of egf accelerates the rate of epidermal regeneration of partial-thickness wounds a...

expression of eukaryotic proteins in e. coli often results in their aggregation. proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. this is not achieved in cytoplasmic expression in e. coli because of the absence of disulfide bonds formation. a novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...

Neurotrophic effects of CS23, a mutein of human basic fibroblast growth factor (bFGF), and recombinant human epidermal growth factor (hEGF) were compared in dissociated cerebellar cultures from embryonic (E20) and early postnatal (P0-5) rats. Both CS23 and hEGF promoted the survival of cerebellar neurons without distinction of ages of animals used. The maximum effects of CS23 were always larger...

Immunohistochemical study showed selective localisation of human epidermal growth factor (hEGF) to the synovial lining layer. Although the synovial lining layer of the rheumatoid, osteoarthritic, and traumatic joints was hEGF positive, hEGF staining was especially dense at the rheumatoid synovial lining layer; the staining increasing linearly according to the degree of stratification of the lin...

The combination of a chitosan 3D-printed scaffold with hydrogel matrix containing an elastin-like polypeptide functionalized the epidermal growth factor (HEGF) was evaluated as possible strategy to obtain bioactive platform stimuli-responsive properties. We designed chitosan/HEGF hybrid and examined physico-chemical properties in vitro behavior when contact simulated biological fluids. Primary ...

Epidermal growth factor (EGF), a polypeptide hormone originally discovered in the mouse submaxillary gland, stimulates growth in a variety of tissues in several species. This hormone has recently been identified in human urine. Herein, radioimmunoassay (RIA) for human EGF (hEGF) has been developed, using human recombinant EGF as reference standard and radioiodinated tracer and antibodies raised...

background epidermal growth factor (egf) plays a fundamental role in the healing of wounds relating to skin damage, the cornea, and the gastrointestinal tract. objectives the aim of this study is the cloning, expression, and purification of recombinant human egf (rhegf), and an assessment of its activity. materials and methods in the present experimental study, a synthetic pet28a (+) -hegf cons...

Human epidermal growth factor (hEGF) has previously been isolated from urine and probably is identical to human beta-urogastrone (hUG). Immunoreactive hEGF/UG has been found in the plasma of normal subjects. In this study, using immunoaffinity chromatography to extract hEGF/UG from plasma, we found that immunoreactive hEGF/UG in blood was associated with blood platelets. It was present in plate...