the key role of chemokine receptor cxcr4 in the maintenance of stemness property of stem cells has been shown recently. the low level laser irradiation (llli) is being used currently in a wide variety of clinical cases as a therapeutic tool for wound healing, relieving pain and destroying tumor cells. the aim of this study was to evaluate the effect of llli mimicking low level laser therapy (ll...

BACKGROUND Human embryos produced by in vitro fertilization (IVF) are usually cultured to day 6 after insemination, and good quality of embryos should develop to blastocyst stage at days 5 and 6. However, some embryos develop slowly, thus they may form blastocysts on day 7. Most IVF laboratories culture embryos to day 6 and then discard retarded embryos that do not develop to blastocyst stage. ...

The key role of chemokine receptor CXCR4 in the maintenance of stemness property of stem cells has been shown recently. The low level laser irradiation (LLLI) is being used currently in a wide variety of clinical cases as a therapeutic tool for wound healing, relieving pain and destroying tumor cells. The aim of this study was to evaluate the effect of LLLI mimicking low level laser therapy (LL...

background: a decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. as co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in human embryos with aneuploidies. materials and methods: in this cohort study, we used fluorescence...

BACKGROUND Cleavage stage embryos as well as postimplantation embryos have been studied extensively over the years. However, our knowledge with respect to the chromosomal constitution of human embryos at the blastocyst stage is still rudimentary. METHODS In the present paper, a large series of human blastocysts was examined by means of fluorescent in situ hybridization (FISH). RESULTS It wa...

background: the selection of pre-embryos for transferred is based on morphological appearance. but some poor quality cleaved embryos also can be cultured to the blastocyst stage and implanted. objective: to assess the clinical pregnancy outcomes of blastocyst transfer which developed from poor quality embryos. materials and methods: a total of 109 cleaved embryos with poor quality were cultured...

Background A decrease in aneuploidy rate following a prolonged co-culture of human blastocysts has been reported. As co-culture is not routinely used in assisted reproductive technology, the present study aimed to evaluate the effect of the prolonged single culture on the rate of diploid cells in human embryos with aneuploidies. MaterialsAndMethods In this cohort study, we used fluorescence in ...

BACKGROUND The early preimplantation embryo relies on mRNA and protein from the oocyte to detect DNA damage and activate DNA repair, cell cycle arrest or apoptosis. Expression of some repair genes has been detected in mammalian oocytes and embryos; however, little is known about DNA repair gene expression in human blastocysts. In this study, DNA repair gene expression was investigated in human ...

In human in-vitro fertilization (IVF), embryos are routinely transferred to the uterus on either day 2 or day 3 of development, resulting in a 10-15% implantation rate. However, in other mammalian species, the transfer of cleavage stage embryos, which normally reside in the oviduct, to the uterus results in a significantly lower implantation rate compared with blastocysts. It is therefore propo...