purpose: to evaluate the effect of human amniotic fluid (haf) on the growth of human corneal endothelial cells (hcecs) and to establish an in vitro method for the expansion of hcecs. methods: hcecs were cultured in dmem-f12 supplemented with 20% fetal bovine serum (fbs). confluent monolayer cultures were trypsinized and passaged using 20% fbs- or 10%, 20%, or 30% haf-containing media. cell prol...

background: adhesion is a common complication of gynecology surgery so different barrier agents and solutions have been used during these operations to separate and protect tissues from adhesion after surgery. adept is one of these solutions that have been postulated to reduce the chance of adhesion following gynecolgy surgery. objective: to evaluate the effect of 4% icodextrin in reducing adhe...

amniotic fluid volume (afv) is one of the important parameters in the assessment of fetal well-being. the ability of ultrasound measurements to represent the actual afv is unproven. this study was undertaken to compare correlation of conventional amniotic fluid index (afi) and radial amniotic fluid index (rafi) as a new method with actual fluid volume on phantom. as an experimental study, 10 to...

stem cells, the last of which can be obtained from fetal tissues, including amniotic fluid [5]. Analyses of human amniotic fluid have been used as safe and reliable screening tools for fetal genetic and congenital diseases for many years [6]. Amniotic fluid contains cells originating from both embryonic and extraembryonic tissues (amnion, placenta, fetus skin, and respiratory, gastrointestinal,...

Studies using in vitro analysis have shown that the interaction between pulmonary surfactant and vernix caseosa could explain the appearance of amniotic fluid turbidity. That phenomenon is interpreted based on the "roll-up" hypothesis. We tested the roll-up hypothesis by examining the presence of micelles of pulmonary surfactant in human amniotic fluid at term. Amniotic fluid samples were colle...

During the second and third trimesters increasing activities of the glycolytic enzyme pyruvate kinase are to be found in human amniotic fluid. At term (37 to 42 weeks), a very steep rise in amniotic fluid pyruvate kinase can be noticed, which may serve as an additional prenatal indicator of fetal maturity. The presumable main sources of the enzyme in amniotic fluid are the epithelium cells of t...

background: cadmium is an extremely toxic metal with ubiquitous environmental distribution. cadmium is a potent reproductive and developmental toxic agent, and intensive environmental exposure to this substance has been reported among the residents of tehran. in this study, we investigated the correlation between amniotic fluid cadmium levels and the risk of preeclampsia, prematurity and low bi...

Eight fatty acids (C12-C18) were previously identified in human amniotic fluid, colostrum, and milk in similar proportions but different amounts. Amniotic fluid is well known to be the natural environment for development in mammals. Interestingly, amniotic fluid and an artificial mixture of fatty acids contained in amniotic fluid produce similar anxiolytic-like actions in Wistar rats. We explor...