background and aims: in this lab trial, the effect of scaffold based on human serum albumin (hsa) and hydroxyapatite nanoparticles (ha nps) on mouse spermatogonial cell line (scl) was investigated. materials and methods: to synthesize ha nps, calcium nitrate and diammonium phosphate at ph 13 were gently added and heated at 100 ºc for 24 hours. then serial concentrations of ha nps was separately...

background: there are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. there are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

in this research, the magnetite nanoparticles (fe304) were prepared by coprecipitation of fe3+ andfe solution in alkaline medium. two kinds of surfactants, cetyl tri methyl ammonium bromide(ctab) and cetyl pyridinum chloride (cpc) were used in the synthesis. fe304 nanoparticles werecoated with human serum albumin (hsa) and bovine serum albumin (bsa). characteristics ofcoated magnetic nanopartic...

advanced glycation end-products (ages) are formed by non-enzymatic reaction between reducing sugar and protein. ages play important roles in pathogenesis of diabetic, aging complications, endothelial dysfunction and neurological diseases such as the alzheimer’s disease. therefore compounds that prevent the glycation reaction are purported to have therapeutic effect on patients with diabetes and...

background and aims: some nanoparticles can be used in immunoassays to increase sensitivity. this study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (bsa nps). materials and methods: at first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. then the concentration of β-subunit of human chorionic gonadotropin (βhcg) i...

background: the immunogenic brucella abortus ribosomal protein l7/l12 is a promising candidate antigen for the development of subunit vaccines against brucellosis. objective: this study was aimed to evaluate the protection of recombinant human serum albumin (has)-l7/l12 fusion protein in balb/c mice. methods: the amplified l7/l12 gene was cloned in pyhsa5 vector, pyhsa5-l7/l12 construct was tra...

human serum albumin (hsa) is the most abundant protein constituent of blood plasma. this protein consists of a single polypeptide chain of 585 amino acid residues, which has many important physiological functions. hsa can bind and carry many drugs, including anticoagulants, tranquilizers, and general anesthetics. some technique such as: fluorescence spectroscopy, three-dimensional fluorescence ...

background glucose is an essential element in the supply of body’s energy. in diseases such as diabetes, glucose increase is associated with disturbance in metabolism. cortisol is an important hormone in the regulation of glucose metabolism, and human serum albumin (hsa) is one of the most important glucose and cortisol transmitters in blood. interaction between albumin and these ligands could ...