Parkinson's disease (PD), caused by selective loss of dopaminergic (DA) neurons in the substantia nigra, is the most common movement disorder with no cure or effective treatment. Exposure to the mitochondrial complex I inhibitor rotenone recapitulates pathological hallmarks of PD in rodents and selective loss of DA neurons in Drosophila. However, mechanisms underlying rotenone toxicity are not ...

Increased oxidative stress is involved in the development of vascular dysfunction and remodeling. Uncoupling protein 2 (UCP2) regulates the production of reactive oxygen species in vascular smooth muscle cells (SMCs). To promote the study of the role of UCP2 in vascular diseases, a transgenic mouse model expressing human UCP2 (hUCP2) in vascular SMCs was established. We constructed a plasmid ca...

BACKGROUND The purpose of this study was to determine whether a 45 bp insertion/deletion (I/D) polymorphism in human uncoupling protein 2 (hUCP2) gene was associated with changes in several cardiovascular risk and physical fitness factors in response to combined exercise during 12 weeks in Korean middle-aged women. The changes in physiological parameters after combined exercise during 12 weeks ...

4 Rection). Polymorphism analysis by Genotyping. Determination of functional properties of the 5’flanking and the promoter region of these two genes: elucidation the 5’ Flanking Region of the hUCP2 gene by PCR-screening a human genomic library. Genome Walking of 5’Flanking Region of human UCP3 Gene. Promoter analysis human UCP –2 /-3 utilizing pCAT-3 reporter gene system, transient transfection...

the purpose of this study was to determine whether a 45 bp insertion/deletion (i/d) polymorphism in human uncoupling protein 2 (hucp2) gene was associated with changes in several cardiovascular risk and physical fitness factors in response to combined exercise during 12 weeks in korean middle-aged women. the changes in physiological parameters after combined exercise during 12 weeks were compar...

Uncoupling proteins 1 (UCP1) and 2 (UCP2) belong to the family of mitochondrial anion transporters and share 59% sequence identity with each other. Whereas UCP1 was shown to be responsible for the rapid production of heat in brown adipose tissue, the primary function and transport properties of ubiquitously expressed UCP2 are controversially discussed. Here, for the first time, the activation p...

Mouse UCP1, human UCP2 and UCP3 were expressed in yeast (Saccharomyces cerevisiae). Total phospholipids were extracted and their fatty acyl composition was analysed. Expression of hUCP2 and hUCP3 significantly altered the mitochondrial phospholipid fatty acyl composition. Palmitoleoyl groups (16:1(n-7)) decreased while oleoyl groups (18:1(n-9)) increased, leading to a significant increase in th...