High-content waste-rubber-modified asphalt (HRMA) has high viscosity and poor storage stability. HRMA not only improves the properties of road asphalt, but also reduces environmental pollution caused by waste tires. Enhancing molecular interaction rubber is key to making full use HRMA. In this paper, aromatic oil was used as activator for rubber. The mechanism between investigated. radial distr...

BACKGROUND High Resolution Melting Analysis (HRMA) is becoming the preferred method for mutation detection. However, its accuracy in the individual clinical diagnostic setting is variable. To assess the diagnostic accuracy of HRMA for human mutations in comparison to DNA sequencing in different routine clinical settings, we have conducted a meta-analysis of published reports. METHODOLOGY/PRIN...

The differentiation of Diplodia pinea from closely related species, such as Diplodia scrobiculata and Diplodia seriata, and its detection in plant tissue, represented a critical issue for a long time. Molecular screening tools have recently been developed to address this topic. In this study we applied one of the most sensitive and rapid diagnostic screening method so far developed, called High...

A new multichannel MAC protocol called Hop Reservation Multiple Access (HRMA) for packet-radio networks is introduced, specified and analyzed. HRMA is based on very-slow frequencyhopping spread spectrum (FHSS) and takes advantage of the time slotting necessary for frequency hopping. HRMA allows a pair of communicating nodes to reserve a frequency hop (channel) using a hop reservation and handsh...

Torque teno viruses (TTVs) are recently discovered DNA viruses, with heterogeneous genomes, highly prevalent in populations worldwide. The species that infect humans are Torque teno virus (TTV), Torque teno midi virus (TTMDV) and Torque teno mini virus (TTMV). High-resolution melting analysis (HRMA) is a sensitive and effective method for genotyping and mutation scanning. Up to now, HRMA has no...

AbsfrucfA new multichannel MAC protocol called Hop-Reservation Multiple Access (HRMA) for wireless ad-hoc networks (multi-hop packet radio networks) is introduced, specified and analyzed. HRMA is based on simple half-duplex, very-slow frequency-hopping spread spectrum (FHSS) radios and takes advantage of the time synchronization necessary for frequency hopping. HRMA allows a pair of communicati...

The hrmA gene of the N2-fixing cyanobacterium Nostoc punctiforme functions in repressing the formation of transitory motile filaments, termed hormogonia, by plant-associated vegetative filaments. Here, we report that anthocyanins can contribute to induction of hrmA expression. Aqueous extract from fronds of the fern Azolla pinnata, a host of symbiotic Nostoc spp., was found to be a potent induc...

Pseudomonas aeruginosa is an opportunistic pathogen that causes nosocomial infections in intensive care units. Determining a system of typing that is discriminatory is essential for epidemiological surveillance of P. aeruginosa. We developed a method for the typing of Pseudomonas aeruginosa, namely, multiple-locus variable-number tandem-repeat (VNTR) typing with high-resolution melting analysis...

BACKGROUND Methodologies like phage display selection, in vitro mutagenesis and the determination of allelic expression differences include steps where large numbers of clones need to be compared and characterised. In the current study we show that high-resolution melt curve analysis (HRMA) is a simple, cost-saving tool to quickly study clonal variation without prior nucleotide sequence knowled...

DNA methylation is a key regulator of gene transcription. Alterations in DNA methylation patterns are common in most cancers, occur early in carcinogenesis and can be detected in body fluids. Reliable and sensitive quantitative assays are required to improve the diagnostic role of methylation in the management of cancer patients. Here we present an optimized procedure, based on differential-hig...