The sensitivity of Helicobacter pylori chromosomal DNA to MboI digestion was investigated in 208 strains from several continents. Only 11 (5%) of strains were sensitive to MboI, and it was hypothesized that HpyIII, a type II restriction/modification enzyme with sequence homology to MboI, mediated the protection. This was confirmed by PCR analysis of the gene locus of hpyIII, normally composed o...

A recently identified Helicobacter pylori gene, hrgA, was previously reported to be present in 70 (33%) of 208 strains examined (T. Ando, T. M. Wassenaar, R. M. Peek, R. A. Aras, A. I. Tschumi, L.-J. Van Doorn, K. Kusugami, and M. J. Blaser, Cancer Res. 62:2385-2389, 2002). Sequence analysis of nine such strains indicated that in each strain hrgA replaced hpyIIIR, which encodes a restriction en...

background : helicobacter pylori is a major human gastric for various gastro duodenal diseases.a number of putative virulence factors such as dupa, homb, tnpa have been described. to date,none were found to be significantly associated with specific h. pylori-related diseases (e.g. gastric cancer and duodenal ulcer). methods : the primary aim of this study was to test the h. pylori hrga genotype...

Received 29 December 2010 Revised 11 January 2011 Accepted 20 January 2011 Early online 21 January 2011 Print 31 January 2011 The recent decades have witnessed an alarming increase in the Helicobacter pylori associated diseases worldwide. In spite of this, deficiencies in our knowledge still exist about its exact epidemiology, the optimum method of its diagnosis and indeed about the precise rol...

Indonesia is one of the countries that has largest nickel reserves in world. In Asian region, Indonesia’s laterite are third position. around 800 thousand tons or about 30% world’s total production, so potential to enjoy demand for this stainless metal [7]. One companies carry out mining Southeast Sulawesi Province PT Gema Kreasi Perdana (GKP). As a newly established company, variety strategies...

Gastric epithelial cells were incubated with a panel of clinical isolates of Helicobacter pylori, including nonulcer dyspepsia with gastritis (HS, n = 20), gastric ulcer (HU, n = 20), duodenal ulcer (HD, n = 21), and gastric cancer (HC, n = 20). HC strains induced a higher cyclooxygenase-2 (COX-2) expression than those from HS, HD, and HU. The bacterial virulence factors and the host cellular p...