Neonatal alloimmune neutropenia (NAIN) is caused by maternal alloimmunisation to fetal human neutrophil antigens (HNAs). This study investigated HNA/HLA alloantibodies involved with NAIN and identified the frequency of in Brazilian neonates. (neutrophil count < 1.5 × 109/L) was samples from 10,000 unselected neonates, resulting 88 neutropenic newborns (NBs) their 83 mothers. Genotyping performe...

The high prophylactic doses of ZnO commonly used to control post-weaning diarrhoea (PWD) in piglets have become an environmental problem. The possibility of partial replacement of ZnO by sodium humate (HNa) in PWD treatment was investigated in this study. Weaned piglets (32) were challenged with two enterotoxigenic E. coli strains (ETEC/O149/F4/LT and ETEC/O147/F18/LT) and allocated into four t...

Among the 5 HNA systems, HNA-3 was the only one that had yet to be characterized. This effort was critical because antibodies directed to HNA-3a are responsible for causing numerous cases of transfusion-related acute lung injury (TRALI), especially fatal TRALI.2-4 Antibodies to HNA-3a cause neutrophil (PMN) agglutination, priming of the PMN oxidase, and PMN-mediated killing of pulmonary endothe...

Human neutrophil antigen 2 (HNA-2) deficiency is a common phenotype as 3-5% humans do not express HNA-2. HNA-2 is coded by CD177 gene that associates with human myeloproliferative disorders. HNA-2 deficient individuals are prone to produce HNA-2 alloantibodies that cause a number of disorders including transfusion-related acute lung injury and immune neutropenia. In addition, the percentages of...

Severe transfusion-related acute lung injury (TRALI) is often due to antibodies in blood components directed against human neutrophil antigen (HNA)-3a. This study aimed to report the genotype frequencies of the HNA-3 system and to estimate the potential risk of HNA-3 incompatibility and alloimmunization in two Thai populations. Eight hundred DNA samples obtained from 500 unrelated healthy blood...

Abstract Genome-wide association studies linked expression of the human neutrophil antigen 3b (HNA-3b) epitope on Slc44a2 protein with a 30% decreased risk venous thrombosis (VT) in humans. is ubiquitous transmembrane identified as receptor for von Willebrand factor (VWF). To explain link between and VT, we wanted to determine how expressing either HNA-3a or HNA-3b neutrophils could modulate th...

Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion-related mortality. Antibodies to HNA-3a are commonly implicated in TRALI. We hypothesized that HNA-3a antibodies prime neutrophils (PMNs) and cause PMN-mediated cytotoxicity through a two-event pathogenesis. Isolated HNA3a or HNA-3a PMNs were incubated with plasma containing HNA-3a antibodies implicated in TRALI, ...

BACKGROUND AND OBJECTIVES Transfusion-related acute lung injury (TRALI) is often caused by antibodies against human neutrophil alloantigen-2 (HNA-2) and HNA-3a. Neutrophil aggregation is considered as a major cause of TRALI, but little is known about how HNA antibodies initiate this process. We explored mechanisms involved in neutrophil aggregation induced by HNA-2 and HNA-3a antibodies. MATE...

The aim of this study was to investigate the effects of sodium humate (HNa), applied to feed or drinking water as a partial substitution for pharmacological doses of ZnO, on clinical, biochemical and haematological indicators of health status, oxidative stress and the serum fatty acid (FA) profile in weaned piglets. Weaned piglets (32) were allocated into four groups: Control = basal diet conta...