Vinasse is ethanol wastewater that still contains nutrients. It can be medium cultivation for C hlorella sp. Cultivation Chlorella in vinasse did not only minimize its COD content but also potentially produce Poly–Hydroxy Butyrate (PHB) a limited nutrient. This paper presents process of on the various was cultivated by adding complete nutrient (urea and TSP), TSP (limited N), urea P). Experimen...