نتایج جستجو برای: his

تعداد نتایج: 180704  

Journal: :Physical chemistry chemical physics : PCCP 2010
Armin Gölzhäuser Christof Wöll

Bending of purple membranes in dependence on the pH analyzed by AFM and single molecule force spectroscopy R.-P. Baumann, M. Schranz and N. Hampp, Phys. Chem. Chem. Phys., 2010 DOI: 10.1039/b919729j Bifunctional polyacrylamide based polymers for the specific binding of hexahistidine tagged proteins on gold surfaces Lucas B. Thompson, Nathan H. Mack and Ralph G. Nuzzo, Phys. Chem. Chem. Phys., 2...

Journal: :Journal of bacteriology 1995
R Rivera-León C J Green B S Vold

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2007
Christina T Hauser Roger Y Tsien

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

2014
Vida Rodríguez Juan A Asenjo Barbara A Andrews

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Journal: :Journal of materials chemistry 2012
Nancy Hom Kinjal R Mehta Tsengming Chou Amy B Foraker Frances M Brodsky Kent Kirshenbaum Jin K Montclare

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

Journal: :Biosensors & bioelectronics 2010
Erman Timurdogan Natali Ozber Sezin Nargul Serhat Yavuz M Salih Kilic I Halil Kavakli Hakan Urey B Erdem Alaca

Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical re...

Journal: :Organic & biomolecular chemistry 2016
Chih-Chi Chu Scott K Silverman

We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). Howeve...

Journal: :Langmuir : the ACS journal of surfaces and colloids 2014
Gabriel S Longo Monica Olvera de la Cruz Igal Szleifer

We present a molecular theory to study the adsorption of different species within pH-sensitive hydrogel nanofilms. The theoretical framework allows for a molecular-level description of all the components of the system, and it explicitly accounts for the acid-base equilibrium. We concentrate on the adsorption of hexahistidine, one of the most widely used tags in bio-related systems, particularly...

Journal: :Acta crystallographica. Section F, Structural biology and crystallization communications 2007
Akari Shinohara Mayuko Sakuma Toshiharu Yakushi Seiji Kojima Keiichi Namba Michio Homma Katsumi Imada

The polar flagellum of Vibrio alginolyticus is rotated by the sodium motor. The stator unit of the sodium motor consists of four different proteins: PomA, PomB, MotX and MotY. MotX and MotY, which are unique components of the sodium motor, form the T-ring structure attached to the LP ring in the periplasmic space. MotY has a putative peptidoglycan-binding motif in its C-terminal region and MotX...

2011
Ryan Choi Angela Kelley David Leibly Stephen Nakazawa Hewitt Alberto Napuli Wesley Van Voorhis

The recombinant expression of soluble proteins in Escherichia coli continues to be a major bottleneck in structural genomics. The establishment of reliable protocols for the performance of small-scale expression and solubility testing is an essential component of structural genomic pipelines. The SSGCID Protein Production Group at the University of Washington (UW-PPG) has developed a high-throu...

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