نتایج جستجو برای: hirudin variant 3

تعداد نتایج: 1893080  

Journal: :research in pharmaceutical sciences 0
seyed mehdi sajjadi 1 cellular and molecular research center, birjand university of medical sciences, birjand, i.r. iran. hamzeh rahimi 2molecular medicine group, biotechnology research center, pasteur institute of iran, tehran, i.r. iran. saeed mohammadi 3hematology, oncology and stem cell transplantation research center, tehran university of medical sciences, tehran, i.r. iran. mohammad faranoush 4rasoul akram medical center, iran university of medical sciences, tehran, i.r. iran. hasan mirzahoseini 5biotechnology group, biotechnology research center, pasteur institute of iran, tehran, i.r. iran. gholamreza toogeh 6thrombosis and homeostasis research center, imam khomeni hospital complex, tehran university of medical sciences, tehran, i.r. iran.

hirudin is an anticoagulant agent of the salivary glands of the medicinal leech. recombinant hirudin (r-hir) displays certain drawbacks including bleeding and immunogenicity. to solve these problems, cysteine-specific pegylation has been proposed as a successful technique. however, proper selection of the appropriate cysteine residue for substitution is a critical step. this study has, for the ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1986
R P Harvey E Degryse L Stefani F Schamber J P Cazenave M Courtney P Tolstoshev J P Lecocq

Cloned cDNAs have been isolated that encode a variant of hirudin, a potent thrombin inhibitor that is secreted by the salivary glands of the medicinal leech, Hirudo medicinalis. This variant probably corresponds to a form that has been purified from leech heads but differs in amino acid sequence from the hirudin purified from whole leeches. There are at least three hirudin transcripts detectabl...

Journal: :Biochemistry 1994
T Szyperski W Antuch M Schick A Betz S R Stone K Wüthrich

Recombinant desulfatohirudin retains largely the thrombin-inhibitory activity of natural hirudin from Hirudo medicinalis and causes at most minimal immune response in humans. With regard to potential pharmaceutical applications it is of interest to further investigate the structural basis of hirudin functions. In this paper transient hydrogen bonds between backbone amide protons and side-chain ...

Journal: :Blood 1997
S Syed P D Schuyler M Kulczycky W P Sheffield

In this study we sought to extend the plasma half-life while maintaining the potent antithrombin activity of hirudin. We hypothesized that gene fusion of hirudin to albumin would result in the expression of a slowly cleared hirudin molecule. A hirudin variant 3 (HV3) cDNA was obtained by gene synthesis, while a 1,996-bp full-length rabbit serum albumin (RSA) cDNA was selected from a rabbit live...

Journal: :The Biochemical journal 1993
J Bichler J W Baynes S R Thorpe

The metabolic fate of the anticoagulant protein, hirudin, and its complex with thrombin are presently unknown. Therefore we have labelled hirudin and human thrombin-hirudin complex with the residualizing label dilactitol-125I-tyramine (*I-DLT) in order to identify their tissue sites of catabolism in the rat. The rapid plasma clearance of hirudin after intravenous injection was unaffected by *I-...

Journal: :Cancer research 1991
N Esumi D Fan I J Fidler

Recombinant desulfatohirudin (r-hirudin), a highly specific inhibitor of thrombin, was examined to determine whether it would inhibit production of experimental lung metastasis by B16-F10 melanoma cells. In in vitro assays using mouse plasma, the high level of procoagulant activity in B16-F10 cells was significantly inhibited by r-hirudin in a dose-dependent manner. From 15 to 120 min after s.c...

Journal: :The New England journal of medicine 1995
P W Serruys J P Herrman R Simon W Rutsch C Bode G J Laarman R van Dijk A A van den Bos V A Umans K A Fox

BACKGROUND The likelihood of restenosis is a major limitation of coronary angioplasty. We studied whether hirudin, a highly selective inhibitor of thrombin with irreversible effects, would prevent restenosis after angioplasty. We compared two regimens of recombinant hirudin with heparin. METHODS We randomly assigned 1141 patients with unstable angina who were scheduled for angioplasty to rece...

Journal: :Circulation 1993
P Zoldhelyi M W Webster V Fuster D E Grill D Gaspar S J Edwards C F Cabot J H Chesebro

BACKGROUND Because the specific antithrombin hirudin prevents platelet-rich arterial thrombus and accelerates thrombolysis in a variety of animal models, it has promise as antithrombotic therapy. We therefore studied the half-life, effect on anticoagulant parameters, and safety of hirudin in patients with coronary artery disease. METHODS AND RESULTS Thirty-eight men and 1 woman (age [mean +/-...

2017
Seyed Mehdi Sajjadi Hamzeh Rahimi Saeed Mohammadi Mohammad Faranoush Hasan Mirzahoseini Gholamreza Toogeh

Hirudin is an anticoagulant agent of the salivary glands of the medicinal leech. Recombinant hirudin (r-Hir) displays certain drawbacks including bleeding and immunogenicity. To solve these problems, cysteine-specific PEGylation has been proposed as a successful technique. However, proper selection of the appropriate cysteine residue for substitution is a critical step. This study has, for the ...

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