HiNF-P and its cofactor p220(NPAT) are principal factors regulating histone gene expression at the G(1)-S phase cell cycle transition. Here, we have investigated whether HiNF-P controls other cell cycle- and cancer-related genes. We used cDNA microarrays to monitor responsiveness of gene expression to small interfering RNA-mediated depletion of HiNF-P. Candidate HiNF-P target genes were examine...

HiNF-P and its cofactor p220 are principal factors regulating histone gene expression at the G1-S phase cell cycle transition. Here, we have investigated whether HiNF-P controls other cell cycle– and cancer-related genes. We used cDNA microarrays to monitor responsiveness of gene expression to small interfering RNA–mediated depletion of HiNF-P. Candidate HiNF-P target genes were examined for th...

At the G(1)/S phase cell cycle transition, multiple histone genes are expressed to ensure that newly synthesized DNA is immediately packaged as chromatin. Here we have purified and functionally characterized the critical transcription factor HiNF-P, which is required for E2F-independent activation of the histone H4 multigene family. Using chromatin immunoprecipitation analysis and ligation-medi...

Genome replication in eukaryotic cells necessitates the stringent coupling of histone biosynthesis with the onset of DNA replication at the G1/S phase transition. A fundamental question is the mechanism that links the restriction (R) point late in G1 with histone gene expression at the onset of S phase. Here we demonstrate that HiNF-P, a transcriptional regulator of replication-dependent histon...

FUNCTIONAL AND STRUCTU CHACTERIZATION OF A HU H4 HISTONE GENE PROMOTER Kenneth Lynn wright November 1990 Thesis Advisor: Janet L. Stein Department: Cell Biology Expression of the cell cycle dependent FOI08 human H4 histone gene is regulated at both the transcriptional and post-transcriptional levels. We have investigated the promoter elements mediating the transcriptional aspects of its regulat...

The deoxytetradecamer d(AAAACGACGGCCAG) has been shown to be an excellent universal primer for sequence determination of DNA cloned into the bacteriophage M13 mp7, mp8, and mp9 series. This new primer offers several advantages over others currently available and it has been used to define the cloning of Hinf I fragments of bacteriophage S13 DNA into the Eco RI site of M13 mp7, utilizing the hom...

Bathymetry on continental hydrographic networks is up to now limited to small areas. This hampers exhaustive hydrological or ecological studies on hydrographic networks at management scales. To enlarge rivers bathymetry surveys, bathymetric, i.e. green LiDAR appears as an interesting tool. But if this technique has been widely used for coastal surveys, very few studies focus on water depth meas...

The objective of the study was to genotype Pandharpuri buffalo breeds for k-casein type by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A 379-bp k-casein gene fragment was amplified using primers K1 (5’CAC GTC ACC CAC ACC CAC ATT TAT C-3’) and K2 (5’-TAA TTA GCC CAT TTC GCC TTC TCT GT-3’). DNA from 20 Pandharpuri buffaloes were extracted by phenol chloroform me...

The objectives of this study were to confirm a location of the calpastatin (CAST) gene in chromosome 2 and to detect associations of genetic variations with economic traits in the porcine CAST gene as a candidate gene for growth and meat quality traits in pigs. Calpastatin is a specific endogenous inhibitor of calpains. The calpain protease system is ubiquitous, and is involved in numerous grow...