a stability indicating high-performance liquid chromatography (hplc) method wasvalidated and used to study the degradation of cetirizine dihydrochloride in acidic and oxidativeconditions. the separation was carried out on a symmetry c18 column and a mixture of 50 mmkh2po4 and acetonitrile (60:40 v/v, ph = 3.5) was used as the mobile phase. the method waslinear over the range of 1-20 μg/ml of ce...

background and objectives:drimia maritima (l.) stearn also known as squill is a medicinally important plant that has been used for various ailments such as dropsy, respiratory disorders, jaundice and epilepsy from ancient times. proscillaridin a is identified as one of the most effective compounds in the plant with remarkable pharmacological features including efficacy against congestive heart ...

dispersive liquid-liquid microextraction method (dllme) combined with high-performance liquid chromatography-ultraviolet detection (hplc-uv) was used to determine thiamine (b1), nicotinamide (b3) and pyridoxine (b6) in sour cherry juice. this method was rapid, simple and sensitive. separation was accomplished using a c18 column. the optimum chromatographic conditions were found to be: mobile ph...

background : doogh is a fermented beverage in which microbial spoilage is possible. benzoic acid and its salt (sodium benzoate) are added to doogh as antimicrobial preservative to control microbial spoilage. however, the use of this preservative has been prohibited in milk and dairy products such as food and drug administrationand institute of standards and industrial research of iran. thus, th...

a high performance liquid chromatography method was studied for determination of 18 ?-glycyrrhetinic acid in glycyrrhiza glabra l. (licorice) extract. the operating condition were c-18 reversed phase column (vp-ods, (250×4.6 mm, 5 mm)) at room temperature, acetonitril/phosphoric acid (3/1) as mobile phase, at flow rate of 0.6ml/min (0-8min), 0.4ml/min (8-20min) and uv detection at 230 nm. the r...

background and objectives:  high concentration of preservatives in food may result in gastrointestinal disturbances whereby some patients suffering from asthma, rhinitis, or urticaria. the aim of this study is the introduction and optimization a new method for simultaneous determination of four preservatives (sb, ps, mp, pp) in foodstuff by high performance liquid chromatography. materials and ...

Submit Manuscript | http://medcraveonline.com Abbreviations: NMR: Nuclear Magnetic Resonance; HPLC: High Performance Liquid Chromatography; RP-HPLC: Reverse Phase High Performance Liquid Chromatography; HPTLC: High Performance Thin Liquid Chromatography; TLC: Thin Liquid Chromatography; API’s: Active Pharmaceutical Ingredient’s; LOD: Limit of Detection; LOQ: Limit of Quantitation; FTIR: Fourier...

the efficacy of activation methods and coupling were studied in the context of performance in batch and fixed bed binding experiments utilizing cell culture fluids or blood plasma as feedstock. conclusions were drawn regarding selection of solid phase according to pore size, rigidity, ph stability, chemistry of derivation and activation, and gross concentration of immobilized ligand required fo...

background: a simple and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been evaluated for the assignment of clonidine hydrochloride in human plasma. methods: the mobile phase composed of acetonitrile-water 60:40 (v/v) and 0.2% formic acid 20 µl of sample was chromatographically analyzed using a repacked zorbax-xdb-ods c18 column (2.1 mmx30...