plant fatty acyl-acyl carrier protein thioesterase (fat) is a major enzyme regulating the amount and composition of fatty acids in lipids. in this study, one type of cdna, corresponding to the fatty acyl-acyl carrier protein (acyl-acp) thioesterase (fat) enzyme, was isolated from the seed of brassica napus cv. ningyou12. blast results revealed that the cdna identified highly with the fatb class...

Background: Corynebacterium glutamicum (C. glutamicum) is a potential host for the secretory production of the heterologous proteins. However, to this date few secretion-type gene expression systems in C. glutamicum have been developed, which limit applications of C. glutamicum in a secretory production of the heterologous proteins.Objectives: In this stu...

Background: Human Cu/Zn superoxide dismutase (hSOD1) is an antioxidant enzyme with potential as a therapeutic agent. However, heterologous expression of hSOD1 has remained an issue due to Cu2+ insufficiency at protein active site, leading to low solubility and enzymatic activity.Objectives:The effect of co-expressed human copper chaperone (hCCS) to enhance the solubility and enzymatic act...

Background: Escherichia coli is still the common host for ing and heterologous protein expression. Various strategies have been employed to increase protein expression in E. coli, but, it seems that external factors such as selection marker concentration can drastically aff ect the yield of protein and plasmid.Objectives: Alterations of protein expression...

results escherichia coli phytase was expressed in p. pastoris under different cultivation conditions (post-induction temperature, methanol concentration, and post-induction ph). the optimized conditions by rsm using face centered central composite design were 1% (v/v) methanol, ph = 5.8, and 24.5°c. under the optimized conditions, appa was successfully expressed in p. pastoris and the maximum p...

Metabolic engineering of microbial hosts for the production heterologous metabolites and biochemicals is an enabling technology to generate meaningful quantities desired products that may be otherwise difficult produce by traditional means. Heterologous metabolite can restricted accumulation toxic within cell. Efflux transport proteins (transporters) provide a potential solution facilitate expo...

Background: The advent of recombinant DNA technology has facilitated heterologous expression of proteins from various sources in different host systems including Escherichia coli. If a plant virus coat protein is expressed in the bacterium it can be used as the antigen for antibody preparation. Such a recombinant antigen preparation can be particularly useful where equipment such as ultracentri...

A Case of Malignant Mixed Ovarian Tumor (OMMMT) with synchronous mesenteric localization presenting heterologous expression neuroendocrine markers, mesothelin and TTFI is studied. The peculiarity this case that immunophenotypic characters not yet reported in the literature OMMMT.