conclusions we introduced an anti-hbsp antibodies elisa, designed for locally circulating hbv strains. correlation observed of anti-hbsp with liver fibrosis staging regardless of viral replication and liver inflammation suggests anti-hbsp antibodies as possible indicator for hbv-associated liver fibrosis. results seven out of eighty (9%) chb patients were positive for anti-hbsp antibodies. mean...

abstract       background and objective: hepatitis b virus (hbv) is a dna virus with high tendency toward hepatic tissue. there are currently about 3 million hbv-infected people and 350 to 400 million chronic carriers of this virus in the world. x protein plays a role in the over-expression of oncogenes, carcinogenicity of liver cells and overlaps with the basal core promoter of the virus. muta...

â  hepadnaviruses employ an unusual strategy for the production of enormous number of virions during replication which makes rapid and substantial genetic sequence changes and alterations. the pathogenesis and clearance of hepatitis b virus (hbv) infection are engaged by the selection and expression of viral mutants durâ­ing virus-host interactions. mutations in regulatory regions such as the b...

background: the hbv-x (hbx) protein is believed to contribute to the development of hcc. however, the molecular mechanisms involved in hbx-mediated hepatocarcinogenesis remain obscure. in this study, the effect of hepatitis b virus x gene and its protein product hbxag on expression of p53 gene in hep g2 cell line was investigated. methods: viral dna extracted from hbv-positive serum and hbx gen...

Replication of the hepatitis B virus is suppressed by deficiency of the X protein. Although several molecules that block cellular targets of X protein reduce the production of hepatitis B virus progeny, the effect of a specific inhibitor of X protein on viral replication has not been investigated. To block X protein specifically, we adopted an intracellular expression approach using H7 single c...

background the hepatitis b virus x (hbx) protein has long been recognized as an important transcriptional transactivator of several genes. human aldo-keto reductase family 1, member c1 (akr1c1), a member of the family of akr1cs, is significantly increased in hbx-expressed cells. objectives this study aimed to investigate the possible mechanism of hbx in regulating akr1c1 expression in hepg2.2.1...

DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...