2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) is a by-product o f t he trichlorophenol (herbicide) production and is also produced through a variety of combustion processes. it appears that tcdd is a ubiquitous chemical, particularly in industrialised countries, and the liver is the target organ of its toxicity. the prominent hepatotoxic effect of tcdd is progressive centrilobular necrosis. the is...

liver injury induced by viruses, chemicals and drugs can be protected by different medicinal plants. feijoa sellowiana (myrtaceae) is an evergreen bush native to southern areas of south america, as well as iran where the fruits are very popular. feijoa has shown a potent antimicrobial effect. morever, the antioxidant activity of total feijoa extract has also been reported. mdma or ecstasy is a ...

We studied the role of copper as a potential mediator of postischemic reperfusion injury in the isolated, perfused rat heart. Hearts were equilibrated with Krebs-Henseleit buffer for 10 minutes and then loaded with copper by way of perfusion with buffer containing 20 pM copper(II)-bis-histidial for 30 minutes. Control hearts were perfused with Krebs-Henseleit buffer alone during the loading per...

Metabolic studies using the in vitro non-recirculating blood-perfused isolated heart model require large volumes of blood. The present study was designed to determine whether heterologous pig blood collected from a slaughterhouse can be used as perfusate for isolated pig hearts perfused under aerobic and constant reduced flow conditions. Eight isolated working pig hearts perfused for 90 min at ...

BACKGROUND The Krebs-Henseleit buffer is the best perfusion solution for isolated mammalian hearts. We hypothesized that a Krebs-Henseleit buffer-based cardioplegic solution might provide better myocardial protection than well-known crystalloid cardioplegic solutions because of its optimal electrolyte and glucose levels, presence of buffer systems, and mild hyperosmolarity. METHODS Isolated L...

OBJECTIVE We previously showed that arrest with multidose infusions of high-dose (1 mmol/L) esmolol (an ultra-short-acting beta-blocker) in oxygenated Krebs-Henseleit buffer (esmolol cardioplegia) provided complete myocardial protection after 40 minutes of normothermic (37 degrees C) global ischemia in isolated rat hearts. In this study we investigated the importance of oxygenation for protecti...

Abbreviations: Bio-LC-ATB-BMPA, 4,4-O-[2-[ 2-[2-[2-[2-[6-(biotinylamino)hexanolyl]amino]ethoxy]exoxy]ethoxy]-4-(1-azi2,2,2-trifluoroethyl)benzoyl]amino-1,3-propanediyl]bis-D-mannose; HES, HEPES, EDTA sucrose; KRH buffer, Krebs-Ringer Hepes buffer; KHB, Krebs-Henseleit bicarbonate; PBS, phosphate-buffered saline; TBS-T, Tris-buffered saline, 0.1% Tween; Carboxy-SNARF-1, 5-(and 6-)carboxy-semi-na...

T-2 toxin, a trichothecene mycotoxin, is considered to be one of the most toxic compounds that are produced by molds, particularly the Fusarium species. Fusarium species have been recognized as a great agricultural problem. They occur worldwide on a variety of plant hosts and cereal grains. The aim of this study was to investigate T-2 toxin-induced liver injury using in situ perfused rat liver....

the isolated perfused rat liver (iprl) model has been used into toxicology study of rat liver. this model provides an opportunity at evaluation of liver function in an isolated setting. studies showed that cd, in a dose-dependent manner, induced toxic effects in iprl models, and these effects were associated with aminotransferase activity and lipid peroxidation. the aim of this study was to inv...