bovine leukosis virus (blv) is a retrovirus that primarily affects lymphoid tissue of cattle. to determine the seroprevalence of enzootic bovine leukosis virus infection and its effects on some clinical, hematological and flow cytometric indications, this study was conducted on 197 holstein cattle slaughtered in an abattoir in tehran province. elisa gp51 test of the obtained serums of the anima...

AIM In India, dairy industries are important for the livelihood of small scale farmers and dairy owners. Tropical theileriosis, mostly affecting dairy cattle and buffaloes is a major threat to dairy and related industries. Tropical theileriosis is caused by Theileria annulata, a hemoprotozoan parasite transmitted by Ixodid ticks of Hyalomma spp. In the present study, we examined the clinical si...

BACKGROUND At present, immunophenotyping of hematological malignancies represents one of the most relevant clinical applications of flow cytometry. In recent years, its use has extended from clinical research to diagnostic laboratories. The aim of this report is to critically review the type of information provided by the flow cytometric immunophenotyping of hematological malignancies and its c...

The most common hematological malignant diseases of the childhood are acute lymphocytic leukemia (ALL) and acute myeloid leukemia (AML). Minimal residual disease is gaining importance nowadays both for therapy efficacy follow up and relapse risk estimation. Owing to fast technological developments, minimal residual disease (MRD) can be evaluated both by molecular and flow cytometric methods, pr...

Flow cytometric immunophenotyping remains an indispensable tool for the diagnosis, classification, staging, and monitoring of hematologic neoplasms. The last 10 years have seen advances in flow cytometry instrumentation and availability of an expanded range of antibodies and fluorochromes that have improved our ability to identify different normal cell populations and recognize phenotypic aberr...

During the past two decades, flow-cytometric immunophenotyping of lymphocytes has evolved from a research technique into a routine laboratory diagnostic test. Extensive studies in healthy individuals resulted in detailed age-related reference values for different lymphocyte subpopulations in peripheral blood. This is an important tool for the diagnosis of hematological and immunological disorde...

background: various fixation and permeabilization techniques have been developed for detection of intracellular antigens by flow cytometry; however, there are few studies using flow cytometry to detect the frequency of intracellular nucleic acids, particularly rna. we tested six different permeabilization methods in order to gain access to a high quality method with minimal damage to intracellu...

Hematopoietic stem cell transplantation (HSCT) has been practiced for more than 30 years. Hematological malignanciesare the main indications for this treatment. However, its indications in adults are different from children.Advances in chemotherapy and target therapy have improved treatment outcome of some of the very high riskcancers, and changed indications of HSCT in children. Multi-center c...