background permanent antigenic variation of influenza viruses causes a major concern to develop an effective human influenza vaccine. conserved antigens are new vaccine candidates because it is not necessary to match the prepared vaccine with circulating strains. ion channel m2 protein is conserved among all influenza a viruses, allowing the virus to enter host cells. objectives to prepare an e...

bacterial and viral triggers are suspected agents in the initial etiology of autoimmune diseases. there are some studies on the etiology of autoimmune disorders which have focused on streptococcal infection and a possible relation with microbial heat shock proteins (hsp) which show significant homology with human heat shock proteins. in addition, some serotypes of streptococci cross-react with ...

recombinant human interferon alpha 2a (rhifn α -2a) production and cell growth were monitored in a set of genetically modified e. coli strains (msd1519, msd1520, msd 1521, msd 1522, msd 1523) producing rhifn α -2a. the growth was followed at od 600 nm, changes in cell physiology were detected by pyrolysis mass spectrometry (pyms) of cell biomass and recombinant protein production was determined...

objective: dendritic cells (dcs) are essential for the activation and polarization of t cells during an adaptive immune response. in this research we investigated the effect of the lymphoide dcs pulsed with heat-treated tumor lysate (htl) as a vaccine in tumor immunotherapy. materials and methods: the balb/c mice were injected subcutaneously in the right flank with wehi-164 fibrosarcoma cells ...

background: heat shock protein 70 (hsp70) is present in all organisms studied so far, and is a major immunogen in infections caused by pathogens including leishmania spp. objective: the aim of this study was to clone and express hsp70 from l. infantum strain mcan/ir/96/lon-49 and evaluate antibody response against hsp70 in visceral leishmaniasis (vl). methods: the l. infantum hsp70 gene segment...

aspergillus fumigatus is a saprophyte fungus, widely spread in a variety of ecologicalniches and the most prevalent aspergilli responsible for human and animal invasiveaspergillosis. the first step to develop novel and efficient therapies is the identificationand understanding of the key tolerance and virulence factors of pathogens. the mainfocus of the present study is to perform the similarit...

background: the aim of this study is to create an ex vivo model to examine the expression of two heat shock protein 70 (hsp70) family members, heat shock protein 72 (hsp72) and heat shock constitute protein 70 (hsc70), at the mrna and protein levels in differentiating corneal cells from air exposed limbal stem cells. materials and methods: limbal biopsies were cultured as explants on a cellular...

Background:  Stresses such as heat shock, starvation, or osmotic is essential to lead isolated microspores towards embryogenesis. Despite the effectiveness of stresses in embryogenesis, they exert adverse effects on metabolism and growth of the regenerated plants. Objectives: The effects of heat shock and 2,4-D treatment on total protein content of treated microspores, morphological and physiol...