Background: Heat shock may affect different aspects of oocyte maturation and its subsequent development to the blastocyst stage. A series of in vitro experiments was performed to determine whether physiologically heat shock (41°C) disrupts the progression of the ovine oocytes through meiosis, activation and blastocyst formation. Materials and Methods: The cumulus-oocyte complexes (COCs) were as...

cacl2 treatment followed by heat shock is the most common method for artificial transformation. here, the cells were transformed using cacl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. the bl21 strain of escherichia coli (e. coli) was being susceptible using cacl2 treatment. some cells were ...

CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain of Escherichia coli (E. coli) was being susceptible using CaCl2 treatment. Some Cells were ...

skeletal muscle may develop adaptive chaperone and enhancementdefense system through daily exercisestimulation. the present study investigated resistance and exhaustion training alters the expression of chaperoneproteins. these proteins function to maintain homeostasis, facilitate repair from injury and provide protection. exercise-induced production of hsps in skeletal muscle and peripheral le...

bacterial and viral triggers are suspected agents in the initial etiology of autoimmune diseases. there are some studies on the etiology of autoimmune disorders which have focused on streptococcal infection and a possible relation with microbial heat shock proteins (hsp) which show significant homology with human heat shock proteins. in addition, some serotypes of streptococci cross-react with ...

conclusions we have highlighted the role of rns3 plus rnt-gp96 mediated by α5integrin in producing il-12 and tnfα. it can be suggested that rnt-gp96 could enhance immunity characteristic of rns3 protein via production of pro-inflammatory cytokines. results our results showed that rnt-gp96 alone significantly increases the expression level of il-12, tnfα and α5integrin in thp-1 macrophages and d...

triploidy in grass carp, ctenopharyngodon idella valenciennes, 1844, was induced on fertilized eggs to compare cold and heat shocks. two simplified methods explained for verification of triploidy in grass carp. the cold shock (7 ?c) was given in three treatments for 30 min starting 2.0, 2.5 and 4.0 min after fertilization. in cold shock, the start point (2.0 min after fertilization) showed the ...

background: heat shock proteins (hsp) are highly conserved molecules with many immunological functions. they are highly immunogenic with important role in cancer immunotherapy and in vaccine development against infectious diseases. as adjuvant, hsp can augment the immunogenicity of weak antigens and can stimulate antigen presenting cells. although vaccines have been successful for many infectio...