AIM Traditional hepatitis B virus (HBV) genotyping methods using restriction fragment length polymorphism (RFLP) can reliably identify genotypes A to F. As HBV genotypes G and H have been recently identified, this study was to establish an accurate and simple genotyping method for all eight HBV genotypes (A to H). METHODS Two hundred and forty HBV small S sequences obtained from GeneBank were...

background and aims: hepatitis b virus (hbv) infects the liver and causes acute and chronic hepatitis, hepatocellular carcinoma and cirrhosis. hbv has been divided to eight genotypes (a–h) and subgenotypes of a, b, c and f. for the first time, we determined hbv genotypes in infected samples by inno-lipa method in yazd, central province of iran. materials and methods: this study was performed on...

Hepatitis B virus (HBV) genotyping has become important in epidemiological and clinical diagnoses, given the relationship between the viral genotype and the progression of disease or the appearance of antiviral resistance. Since genotyping by sequence and phylogenetic analyses is not convenient in the clinical setting, we evaluated InnoLipa HBV genotyping (Innogenetics, Belgium) and an HBV DNA-...

Background and Aims: Hepatitis B virus (HBV) infects the liver and causes acute and chronic hepatitis, hepatocellular carcinoma and cirrhosis. HBV has been divided to eight genotypes (A–H) and subgenotypes of A, B, C and F. For the first time, we determined HBV genotypes in infected samples by INNO-LiPA method in Yazd, central province of Iran. Materials and Methods: This study was performed...

Background and Aims: chronic HBV infection is one of the most common viral infections in worldwide which many factors such as genetic factors are involved in pathogenesis of disease. Gamma interferon (IFN-γ) and its receptor (INFGR) play a critical role in the immune response to HBV infection. Single nucleotide polymorphisms (SNPs) are effective on level of gene expression, The aim of thi...

    Background: Hepatitis B Virus (HBV) causes acute and chronic liver disease worldwide. HBV has eight genotypes (A to H) which is the reflection of its genome with their characteristic geographical distribution. Each genotype could have different pathogenic and therapeutic characteristics. There have been few records on HBV genotyping in general population from our region. This study aimed to...

A fast and low-cost method for HBV genotyping especially for genotypes A, B, C and D was developed and tested. A classifier was used to detect and analyze a one-step immunoassay lateral flow strip functionalized with genotype-specific monoclonal antibodies (mAbs) on multiple capture lines in the form of pattern recognition for point-of-care (POC) diagnostics. The fluorescent signals from the ca...

Background & Aims HBV genotype G (HBV/G) is mainly found in co-infections with other genotypes and was identified as an independent risk factor for liver fibrosis. This study aimed to analyse the prevalence of HBV/G healthy European carriers characterize crosstalk genotypes. Methods A total 560 were tested via HBV/G-specific PCR co-infections. Quasispecies distribution analysed deep sequencing,...

In a country with a high prevalence (16%) of chronic serum HBsAg carriers like Romania there is a special interest in the diagnosis, epidemiology, clinics, pathology and treatment of HBV infection The idea of HBV genotyping arose from the need of understanding the complex interactions between virus and host. The purpose of this article is to present a study which aimed to identify the circulati...

background the occult hepatitis b infection (obi) is defined as the presence of hepatitis b virus (hbv) dna in the sera or in the liver biopsy and the absence of hepatitis b surface antigen (hbsag) by serological test. objectives the current study aimed to evaluate the occult hbv infection by polymerase chain reaction (pcr) and determine hbv genotyping among the patients with abnormal alanine t...