Administration or expression of growth factors, as well as implantation of autologous bone marrow cells, promote in vivo angiogenesis. This study investigated the angiogenic potential of combining both approaches through the allogenic transplantation of bone marrow-derived mesenchymal stem cells (MSCs) expressing human basic fibroblast growth factor (hbFGF). After establishing a hind limb ische...

Neutrophic effects of recombinant human basic fibroblast growth factor (hbFGF) and its modified protein CS23 on brain neurons were evaluated by their abilities to promote survival of primary cultured neurons from various regions of fetal rat brain. In the molecule of CS23, two serines are substituted for two cysteines at positions 70 and 88 in the natural hbFGF. Both hbFGF and CS23 markedly inc...

Effects of recombinant human epidermal growth factor (hEGF) on survival of primary cultured neurons from various regions of fetal rat brain were compared with those of recombinant human basic fibroblast growth factor (hbFGF). Addition of hEGF (0.001-10 ng/ml) in chemically-defined serum-free medium enhanced significantly the survival of cultured neurons from all regions tested, i.e., cerebral c...

PURPOSE Effective therapy for visual loss caused by optic nerve injury or diseases has not been achieved even though the optic nerve has the regeneration potential after injury. This study was designed to modify amniotic epithelial cells (AECs) with basic fibroblast growth factor (bFGF) gene, preliminarily investigating its effect on transected optic nerve. METHODS A human bFGF gene segment w...

Purpose: To improve the stability and bioactivity of human basic fibroblast growth factor (hbFGF) by site-specific pegylation Methods: Four new mutants of hbFGF were designed with substituted Asp68, Lys77, Glu78 and Arg81 with cysteine with the aid of bioinformatics technique, and then cloned into pET21a plasmid, transferred into E. coli BL21 (DE3). The expressed proteins were purified using ca...

chaperones are produced by prokaryotic, yeast and higher eukaryotic cells for various purposes. over-expression of each chaperone or sets of them affect the production level of a recombinant protein in the cell. on the basis of this hypothesis, five different plasmids with 5 different combinations of 6 chaperones molecule, transformed into escherichia coli along with human basic fibroblast grow...

Chaperones are produced by prokaryotic, yeast and higher eukaryotic cells for various purposes. Over-expression of each chaperone or sets of them affect the production level of a recombinant protein in the cell. On the basis of this hypothesis, five different plasmids with 5 different combinations of 6 chaperones molecule, transformed into Escherichia coli along with human basic Fibroblast Grow...

The gene encoding the human basic fibroblast growth factor (hbFGF) has been already chemically-synthesized and cloned in pET-3a expression vector (Pasteur Institute of Iran). In the present study, we compared the level of expression of this synthetic hbFGF and its related cDNA in Escherichia coli. The pBR322-cDNA of hbFGF supplied by Dr. Seno (from Molecular Biology Dept, Okaido prefectural uni...

the gene encoding the human basic fibroblast growth factor (hbfgf) has been already chemically-synthesized and cloned in pet-3a expression vector (pasteur institute of iran). in the present study, we compared the level of expression of this synthetic hbfgf and its related cdna in escherichia coli. the pbr322-cdna of hbfgf supplied by dr. seno (from molecular biology dept, okaido prefectural uni...

embryonic stem cells (escs) are originally derived from the icm of blastocysts and are characterized by their ability to self-renew and their pluripotencies. only a few reports have been published on esc isolations and line establishment in animals, even fewer in horses. however, it is still important to isolate equine escs for animal biotechnology and therapeutic applications. in the present s...