introduction: β-globin gene cluster haplotypes are useful in diagnosis of particular molecular defects in β-thalassemia, prenatal diagnosis of β-thalassemia, and elucidating population affinities. methods: β-globin gene cluster haplotypes were studied in 150 β-thalassemia minor and 52 healthy in-dividuals from the fars province of iran. dna was extracted from leukocytes of whole blood by phe-no...

persian gazelle (gazelle subgutturosa) is one of the most important species in the world with its wide distribution in iran the greatest number of this species in iran is associated with the gazelles in sohrain plain in sorkhabad protected region in zanjan province. considering the significance of this species in the region and the fact that no genetic studies have been conducted to determine t...

common variable immunodeficiency (cvid) is an antibody deficiency syndrome that often co-occurs in families with selective iga deficiency (igad). this study was designed to investigate the frequency of dr and dq loci of hla class ii region in common variable immunodeficiency (cvid) patients. fifteen iranian patients with cvid or igad (mean age 14.6±5.4, range 4-25 years; 9 male and 6 female) an...

Lactoferrin (Lf) is a bioactive protein present in all external secretions such as milk that plays important role in the innate host defense. Therefore, the Lf has a major role in bovine mammary gland defense during mastitis. In this study, 5’-flanking region of bovine Lf gene containing three single nucleotide polymorphisms (SNPs) at positions −602 (T/G), −600 (A/G) ...

Background: Sickle cell anemia is relatively common in Khuzestan province located in Southwest Iran. The characteristics of sickle cell disease in Iran are apparently different from other regions some of these characteristics might be related to β-chain haplotypes. The purpose of this study was to determine the frequency of β-chain haplotypes in 50 patients with homozygous sickle cell anemia in...

PCR-based mtDNA analysis (RFLP) was used for the study of population differentiation in the Russian sturgeon (Acipenser gueldenstaedti). The mtDNA ND5/6 gene regions were amplified using PCR techniques followed by RFLP analysis. 39 different composite haplotypes were detected among 62 specimens. 29 haplotypes were rare occuring only once in two regions (west and east areas of the Southern Caspi...

PCR-based mtDNA analysis (RFLP) was used for the study of population differentiation in the Russian sturgeon (Acipenser gueldenstaedti). The mtDNA ND5/6 gene regions were amplified using PCR techniques followed by RFLP analysis. 39 different composite haplotypes were detected among 62 specimens. 29 haplotypes were rare occuring only once in two regions (west and east areas of the Southern Caspi...

Background: Molecular genetic factors regulating hemoglobin F (Hb F) expression are important modifiers of the severity of sickle cell anemia (SS). Methods: The prevalence of XmnI polymorphic site, the Gg:Ag ratio and the Hb F level were determined using PCR-RFLP procedure, HPLC and alkaline denaturation method, respectively, in various haplotypes of 52 patients with SS, 18 patients with sickle...

The genetic variation and population structure of narrow-clawed crayfish (Astacus leptodactylus) was examined by means of polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) analysis of the cytochrome oxidase subunit I (COI) of mitochondrial DNA. A total of 194 adult specimens were collected from seven sample sites including, two in the south Caspian Sea and one each...

Mitochondria1 DNA (mtDNA) control region sequences were analyzed to evaluate the population genetic structure of Persian sturgeon (Acipenser persicus) in Caspian Sea. A total of 45 specimens were collected from the different locations of the Caspian Sea. MtDNA control region was amplified using PCR. Direct sequencing was performed according standard method. The results showed that 12 haplotypes...