The pathophysiology of Malignant Hyperthermia Susceptibility (MHS) involves abnormal calcium release from the skeletal muscle’s sarcoplasmic reticulum. MHS diagnosis relies on either presence a pathogenic genetic variant in RYR1, CACNA1S, or STAC3, positive vitro caffeine-halothane contracture testing (CHCT). Muscle strips 65% 93 patients diagnosed as by CHCT our clinic developed excessive forc...

background: cirrhotic patients are at a greater risk of different kinds of arrhythmias. despite the well-known arrhythmogenic effects of halothane, developing countries continue to use halothane due to its low cost. this study was designed to investigate the pathophysiology of halothane untoward effects on heart rhythm moreover was aimed to explore the effect of halothane anesthesia on the thre...

conclusions despite the adverse effects of inhalational halogenated anesthetics (especially halothane) on hepatic patients when necessary. the effects on all systems must be considered and the necessary preparations must be provided. these drugs are still used, if necessary, due to the presence of positive effects and advantages mentioned in other studies as well as the adverse effects of other...

The effect of halothane on the physiological response to excitatory stimuli was assessed in clonal (GH3) pituitary cells. Halothane, at concentrations used to produce general anesthesia in animals (0.25-0.76 mM), inhibited thyrotropin-releasing hormone (TRH)-induced prolactin (PRL) secretion. The sustained (extracellular calcium-dependent) phase of PRL secretion was 70 +/- 7% inhibited by the h...

Following use with halothane, ten anaesthestic machines were sampled using infrared analysis for halothane contamination. Baseline measurements of halothane were made in the room and at the machine's common gas outlet. Five per cent halothane with four litres per minute oxygen flow was delivered for ten minutes into a scavenged breathing circuit. Halothane was then discontinued, an oxygen flow ...

Previous work showed widespread saturable binding of halothane in rat brain. To determine whether this represents selective binding to a few widespread proteins or less selective binding to many different proteins, we used [(14)C]halothane photolabeling and quantitative electrophoresis/autoradiography in rat cerebellar homogenates. Many proteins incorporate label. Stoichiometry values ranged fr...

As an index of delayed hypersensitivity in vitro halothane macrophage migration inhibition factor tests (halothane-MIF tests) were performed on peripheral blood lymphocytes from five patients with halothane hepatitis. Twenty-two subjects exposed to halothane, but with no evidence of jaundice, five 'healthy' hospital anaesthetists, nine jaundiced subjects without halothane exposure, and 10 healt...

The effect of halothane on isolated calcium (Ca2+) current of clonal (GH3) pituitary cells was investigated using standard whole-cell clamp techniques at room temperature. Halothane (0.1-5.0 mM) reversibly reduced both the low-threshold, transient [low-voltage-activated (LVA)] component and the high-threshold [high-voltage-activated (HVA)] component of Ca2+ current. Halothane had little effect ...

background: halothane is the routine used inhalation anesthetic drug in the world but at present, there is an increasing interest for using intravenous anesthetics like propofol. the purpose of this study was to compare these anesthetic effects on hepatic enzymes.methods: from 2006 to 2007, this study was performed on fifty eight patients classified in class i of american society of anesthetics...