background: purple coneflower (echinacea purpurea l.) is one of the most important medicinal plants. hairy root culture of echinace, represent a valuable alternative to field cultivation for the production of bioactive secondary metabolites. objective: the present study investigates the effects of kno3, cacl2 and mgso4 concentrations on growth and the biosynthesis of cichoric acid in echinacea ...

Using Agrobacterium rhizogenes due to create hairy roots is a useful method to product secondary metabolites in many medicinal plants. The transgenic hairy roots were induced from Peganum multisectum (Maxim) Bobrov a medicinally important species, by infecting leaf and stem explant with wild type Agrobacterium rhizogenes strain ATTCC 15834, which led to the induction of hairy roots from 19% of ...

silymarin production by hairy root culture of milk thistle (silybum marianum l. gaertn) was investigated using agrobacterium rhizogenes ar15834. hairy roots were induced by injection or inoculation of explants with a. rhizogenes. one month old hairy roots were dissected from the explants and grown in murashing and skoog (ms) liquid medium. polymerase chain reaction (pcr) using the b gene and th...

background and objectives: hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. in the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in hyoscyamus reticulatus. methods: effect of different factors including agrobacteriu...

Literature review shows that hepatitis C infection may increase the risk of non-hodgkin lymphoma. Reactivation of hepatitis B infection has been reported in patients with hairy cell leukemia after chemotherapy and/or splenectomy. We present hepatitis B surface (HBs) antigenemia and concurrent hepatitis C virus (HCV) seropositivity in a case of hairy cell leukemia. Key Words: Hairy cell leukemi...

Hairy roots culture is a potential system for secondary metabolite production in medicinal plants. In this research, hairy root induction in Echinacea angustifolia was established using Agrobacterium rhizogenes. The effects of dif-ferent A. rhizogenes strains (AR15834, A4, K599 and MSU440) in two solid growth regula-tor-free culture media, half strength MS salts with B5 vitamins and WPM, were e...

The (-)-hyoscyamine, atropine and scopolamine (hyoscine) are three valuable tropane alkaloids while scopolamine is the most important member of this group for the pharmaceutical industry due to its higher demand compared to hyoscyamine and atropine. Scopolamine is an anticholinergic reagent with several therapeutic applications. In the current study, the hairy roots culture of Daturametel was u...

Transcriptional repression plays an essential role in many aspects of metazoan development. Drosophila hairy is a primary pair-rule gene encoding a basic helix-loop-helix class transcriptional repressor that is required for proper segmentation. Previous characterization of Hairy-binding proteins has implicated two different classes of histone deacetylase as mediators of Hairy repression. Here, ...

Silymarin production by hairy root culture of Milk thistle (Silybum marianum L. Gaertn) was investigated using Agrobacterium rhizogenes AR15834. Hairy roots were induced by injection or inoculation of explants with A. rhizogenes. One month old hairy roots were dissected from the explants and grown in Murashing and Skoog (MS) liquid medium. Polymerase Chain Reaction (PCR) using the B gene and th...