The responses of liver and serum gluta­mate dehydrogenase (CDII; EC 1.4.1.2.), isocitrate (ICDH; 1.1. 1.42) the urea cycle enzyme, ornithine carbamoyltransferase (OCT; 2. 1.3.3) to dietary lima bean haemagglutinin (lectin) were assessed using a total S4 growing rats. GDH ICDH activities in highly significantly (P < 0.001) eleva­ted by haemagglutinin. activi­ties correlated 0.01) with levels ...

The cDNAs encoding haemagglutinin I from plasmodia of Physarum polycephalum have been cloned using PCR protocols. The composite haemagglutinin I cDNA sequence, derived from several overlapping clones from PCR fragments, spans 408 nt and the 315 bp ORF encodes a polypeptide of 104 aa without a typical signal sequence. The putative molecular mass deduced from the amino acid sequence (10,760.76 Da...

To elucidate inconsistencies in rubella haemagglutinin assays the components of the assay technique were examined. The results of carefully controlled assays of rubella haemagglutinin antigens from different sources in various plates and diluents with four species of indicator cells are reported. The quality and quantity of gelatin in the dextrose-gelatin-veronal buffered diluent commonly used ...

The global outbreak of novel A/H1N1 spread in human population worldwide has revealed an emergency need for producing a vaccine against this virus. Current influenza vaccines encounter problems with safety issues and weak response in high-risk population. It has been established that haemagglutinin is the most important viral antigen ...

The haemagglutinin glycoprotein is the major surface antigen of influenza virus and is responsible for receptor binding, membrane fusion and antigenic variation. The 1968 Hong Kong haemagglutinin contains seven Asn-Xaa-Ser/Thr sequences, all of which are glycosylated (1 3 000daltons of carbohydrate per monomer, 20% carbohydrate by wt.). The three-dimensional structure of the haemagglutinin (Wil...

The role of the Golgi complex in the intracellular transport of influenza virus haemagglutinin in infected MDCK cell monolayers has been investigated using monensin, a carboxylic ionophore known to disrupt the functioning of this organelle in other cell types. In untreated cells metabolically labelled 5 h post-infection with [35S]methionine haemagglutinin was first seen in core glycosylated for...

Forty-five strains of Shigella were screened for haemagglutinin production and broad-spectrum haemagglutination reaction. Mannose-sensitive haemagglutinin (MSHA) was found in 22 strains [Shigella flexneri (7), S. dysenteriae (7), S. sonnei (3), and S. boydii (5)]. Eighteen strains harboured mannose-resistant haemagglutinin (MRHA), and 8 strains were observed to be non-haemagglutinating to guine...

Signalling lymphocyte activation molecule (SLAM) acts as a cellular receptor for Measles virus (MV). The recombinant MV, based on a SLAM-using clinical isolate in which asparagine at position 481 of the haemagglutinin was replaced with tyrosine, was generated. Characterization of this recombinant virus revealed that the N481Y substitution in the haemagglutinin allowed it to utilize CD46 as an a...

It is suggested that Haemophilus paragallinarum requires at least three haemagglutinins for adhesion during infection. This paper reports the partial purification and characterization of the HA-L haemagglutinin from H. paragallinarum strain 46-C3, a heat sensitive, trypsin sensitive haemagglutinin that has been shown to be the serovar specific haemagglutinin in this organism. Using the pl and m...

The amino acid sequence and oligosaccharide distribution for the haemagglutinin from the early Hong Kong influenza virus A/Aichi/2/68 (X-31) was investigated. The two polypeptide chains, HA1 and HA2, were fragmented by CNBr and enzymic digestion, and the amino acid sequence of each small peptide was deduced by comparing its chromatographic behaviour, electrophoretic mobility, amino acid composi...