BACKGROUND/OBJECTIVES The mitochondrial β-oxidation of fatty acids is a complex catabolic pathway. One of the enzymes of this pathway is the heterooctameric mitochondrial trifunctional protein (MTP), composed of four α- and β-subunits. Mutations in MTP genes (HADHA and HADHB), both located on chromosome 2p23, cause MTP deficiency, a rare autosomal recessive metabolic disorder characterized by d...

The pre-diagnostic test for preimplantation genetic diagnosis (PGD) of long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency was performed by polymerase chain reaction (PCR) and direct sequencing for hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (HADHA) gene. We obtained unexpected genotyping results of HADHA gene by allele drop-out in the analys...

Background: This research aimed to investigate the effect of resistance and high-intense interval training consumption grape seed oil on expression HADHA, CPT1, CD36 genes in male rats' gastrocnemius soleus muscles. Methods: Thirty Wistar rats (250 ± 25g) were divided into six groups. The group performed 3 weekly sessions for 8 weeks, high-intensity running a treadmill eight 5 week. (3.7g/kg) w...

BACKGROUND The human mitochondrial trifunctional protein (MTP) complex is composed of 4 hydroacyl-CoA dehydrogenase-alpha (HADHA) and 4 hydroacyl-CoA dehydrogenase-beta (HADHB) subunits, which catalyze the last 3 steps in the fatty acid beta-oxidation spiral of long-chain fatty acids. The HADHB gene encodes long-chain ketoacyl-CoA thiolase (LCTH) activity, whereas the HADHA gene contains the in...

At the 22 meeting of the Committee for Risk Assessment (RAC) in September 2012, the ECHA Secretariat presented a proposal to set DNELs and dose response relationships for substances prior to receiving applications for authorisation (AfAs). This was approved by RAC as a trial exercise. However, in early 2015, ECHA agreed to continue supporting the practice for Annex XIV substances, recognizing i...

We appreciate the interest and discussion our study reassessing animal use and costs of ReACH (Rovida and Hartung, 2009, Hartung and Rovida, 2009) has initiated. the issue has received serious press coverage in several countries. This reflects the extent of the ReACH investment into consumer product safety, and it should stimulate further discussion regarding a revision of safety testing approa...

The torque output accuracy of the IPMSM in electric vehicles using a state of the art MTPA strategy highly depends on the accuracy of machine parameters, thus, a torque estimation method is necessary for the safety of the vehicle. In this paper, a torque estimation method based on flux estimator with a modified low pass filter is presented. Moreover, by taking into account the non-ideal charact...

Rhodnius prolixus is an obligatory hematophagous insect, vector of Chagas disease. After blood meal, lipids are absorbed, metabolized, synthesized, and accumulated in the fat body. When necessary, stored mobilized, transported to other organs, or oxidized provide energy. Mitochondrial ?-oxidation a cyclic conserved pathway, where degradation long-chain fatty acids occurs contribute cellular ene...