Microsomal epoxide hydrolase is a critical biotransformation enzyme that catalyzes the conversion of a broad array of xenobiotic epoxide substrates to more polar diol metabolites. The gene has been shown previously to exhibit polymorphism, including variation in the coding region leading to amino acid substitutions at positions 113 (Y/H) and 139 (H/R). To better evaluate the phenotype associate...

Mitarai, Sayaka, Thomas D. Reed, and Atsuko Yatani. Changes in ionic currents and b-adrenergic receptor signaling in hypertrophied myocytes overexpressing Gaq. Am J Physiol Heart Circ Physiol 279: H139–H148, 2000.—Transgenic overexpression of Gaq causes cardiac hypertrophy and depressed contractile responses to b-adrenergic receptor agonists. The electrophysiological basis of the altered myocar...

Human microsomal epoxide hydrolase (EPHX1) is active in the metabolism of many potentially carcinogenic or otherwise genotoxic epoxides, such as those derived from the oxidation of polyaromatic hydrocarbons. EPHX1 is polymorphic and encodes allelic variation at least two amino acid positions, Y113H and H139R. In a number of recent molecular epidemiological investigations, EPHX1 polymorphism has...

Bacteriophage λ protein phosphatase (λPP) with Mn2+ as the activating metal cofactor was studied using phosphatase inhibition kinetics and electron paramagnetic resonance (EPR) spectroscopy. Orthophosphate and the oxoanion analogues orthovanadate, tungstate, molybdate, arsenate, and sulfate were shown to inhibit the phosphomonoesterase activity of λPP, albeit with inhibition constants (Ki) that...

This study was to investigate if endogenous pituitary adenylate cyclase-activating polypeptide (PACAP) can be released during direct splanchnic nerve stimulation in vivo, and to determine whether PACAP in the adrenal gland can modulate the medullary response to sympathoadrenal reflex. The output of adrenal catecholamine and PACAP38-like immunoreactivity (PACAP-38-ir) increased in a frequency-de...