Guanidine hydrochloride has been widely used in the initial recovery steps of active protein from the inclusion bodies in aqueous two-phase system (ATPS). The knowledge of the guanidine hydrochloride effects on the liquid-liquid equilibrium (LLE) phase diagram behavior is still inadequate and no comprehensive theory exists for the prediction of the experimental trends. Therefore the effect the ...

This report focus on phase behavior of polyethylene glycol (PEG)4000/ phosphate/ guanidine hydrochloride/ water system at different guanidine hydrochloride concentrations and pH. The binodal of the systems was displaced toward higher concentrations of the components with increasing guanidine hydrochloride concentrations. The partition coefficient of guanidine hydrochloride was near unity and in...

This report focus on phase behavior of polyethylene glycol (PEG)4000/ phosphate/ guanidine hydrochloride/ water system at different guanidine hydrochloride concentrations and pH. The binodal of the systems was displaced toward higher concentrations of the components with increasing guanidine hydrochloride concentrations. The partition coefficient of guanidine hydrochloride was near unity and in...

In studies on muscular dystrophy in young individuals investigations of the urine were made along three lines, as follows: (a) the estimation of creatinine and creatine, according to Folin (1) and Benedict (2) respectively; (b) the study as to whether the creatine findings include other substances than creatine; (c) the possibility that in muscular dystrophy guanidine intoxication plays a promi...

Inhibition of HeLa cell deoxyribonucleic acid (DNA) synthesis, which occurred by the 4th to 5th hr after infection with poliovirus, could be blocked completely by guanidine only when it was present before the 2nd hr. At the 2nd hr, there was no significant ribonucleic acid (RNA)-replicase activity, and addition of guanidine inhibited all production of virus but allowed 57% of maximal DNA inhibi...

The z-average molecular weight of horse liver alcohol dehydrogenase in dilute neutral buffer is 78,200 by sedimentation equilibrium. Guanidine hydrochloride, 1 M, reversibly inhibits enzymatic activity. The inhibition with nicotinamide adenine dinucleotide is competitive and with ethanol is mixed. When ethylenediaminetetraacetate is added to the enzyme in 1 M guanidine hydrochloride, zinc is re...

Prior studies of the viscosity of the subunit proteoglycan obtained by dissociative extraction of bovine nasal septum cartilage suggested that the proteoglycan subunit molecule behaves as a rigid rod in aqueous solution over the range of 0.5 to 4.0 M guanidine hydrochloride. A more direct view of dimension and shape can be obtained from light-scattering and viscosity of bovine nasal cartilage p...