نتایج جستجو برای: green flourescent proteins
تعداد نتایج: 674983 فیلتر نتایج به سال:
background: mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. this study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different non-viral transfection procedures. methods: in the current study, calcium phosphate (cap), deae-...
Green fluorescent protein allows gene expression and protein localization to be observed in living cells.
the green fluorescent protein (gfp) was originally isolated from the jellyfish aequorea victoria that fluoresces green when exposed to blue light. gfp protein is composed of 238 amino acids with the molecular mass of 26.9 kd. the gfp gene is frequently used in cellular and molecular biology as a reporter gene. to date, many bacterial, yeast , fungal, plants, fly and mammalian cells, including h...
Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 10(6) pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis. Besides confirmation o...
agrobacterium tumefaciens is capable of gene transfer to both plant and non-plant organisms. indeed, upon infection of eukaryotic cells, agrobacterium tumefaciens transfers a piece of its tumor inducing (ti)-plasmid, called t-dna, to the host cell nucleus, which subsequently integrates into the host genome. the vird2 virulence protein which has relaxase endonuclease activities covalently binds ...
Actin filament assembly is critical for eukaryotic cell motility. Arp2/3 complex and capping protein (CP) regulate actin assembly in vitro. To understand how these proteins regulate the dynamics of actin filament assembly in a motile cell, we visualized their distribution in living fibroblasts using green flourescent protein (GFP) tagging. Both proteins were concentrated in motile regions at th...
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