objective(s): pesticides has wide range of infertility in female reproductive. this study was done to evaluate the effect of movento pesticide on development of granulosa cells and ovarian follicles and foxo1 and vnn1 gene expression in balb/c mice. materials and methods: in this study 40 healthy balb/c mice 5-6 weeks age were used. animals were randomly allocated into four groups. control (wit...

Ovarian follicle atresia is thought to be induced through apoptosis of granulosa cells. This study was designed to investigate the possible involvement of nitric oxide (NO) in granulosa cell apoptosis. In immature rat ovaries obtained 48 h after pregnant mare serum gonadotropin administration, immunohistochemistry and terminal deoxynucleotidyl transferase-mediated dUTPbiotin nick end labeling (...

Follicular atresia is characterized by a rapid loss of granulosa cells and, to a lesser extent, theca cells, via apoptosis. The aim of this study was to investigate the possible involvement of theca cell secretions in the regulation of apoptosis of rabbit granulosa cells. The annexin-V binding method based on externalization of phosphatidylserine to the outer layer of plasma membrane during apo...

Two monoclonal antibodies capable of inducing granulosa cell apoptosis were produced against granulosa cells prepared from antral follicles of pig ovaries. The healthy follicles, 4-5 mm in diameter, were dissected from the ovaries of gilts, and then granulosa cells were isolated. BALB/c female mice were immunized with the isolated granulosa cells. Antibodies against the granulosa cells were det...

background: peroxisome proliferative-activated receptors (ppars) are nuclear receptors that involved in cellular lipid metabolism and differentiation. the subtype ? of the ppar family (ppar?) plays important roles in physiologic functions of ovaries. objective: to determine correlation between ppar? protein level in granulosa cells and pregnancy rate in women undergoing in-vitro fertilization (...

The granulosa cell tumor is the most common malignant functioning tumor of the ovary. When functional, granulosa tumor almost always are estrogenic although rare tumors are androgenic. Progesterone producing by these tumors has only been reported twice. We report a case of granulosa cell tumor in a 7 years old girl with precocious puberty who represent the first case of progesterone producing b...

An increasing number of studies suggest that ornithine decarboxylase antizyme 1 (OAZ1), which is regarded as a tumor suppressor gene, regulates follicular development, ovulation, and steroidogenesis. The granulosa cells in the ovary play a critical role in these ovarian functions. However, the action of OAZ1 mediating physiological functions of granulosa cells is obscure. OAZ1 knockdown in gran...

background: the omega-3 (ω-3) fatty acid eicosapentaenoic acid (epa) is currently used in the clinic as a nutritional supplement to improve infertility, particularly in women with polycystic ovarian syndrome (pcos).objective: the present study was designed to investigate the effect of epa on insulin-like growth factor 1 (igf-1) and cyclooxygenase 2 (cox-2) gene expression in primary cultured gr...

Previously, it has been shown that integrin alpha6beta1 expressed on human granulosa cells regulates luteinization in co-operation with its ligand, laminin. In this study, integrin alpha2 was immunohistochemically demonstrated to be expressed on granulosa and large luteal cells. It was also detected on luteinizing theca interna cells after ovulation. Immunoreactive collagen type IV, which is on...

Granulosa cells support the developing oocytes and serve as transducers of the ovulatory stimulus induced by LH surge. Fyn kinase is expressed in granulosa cells, though its role in these cells has not been studied. In human embryonic kidney 293T cells, microRNA (miR)-125a-3p down-regulates Fyn expression, causing a decrease in cells' migratory ability. Our aim was to explore the role of miR-12...