a new system is presented for the generation ofrecombinant bacillus subtilis strains without antibioticmarkers. this system is based on two plasmids constructed in escherichia coli. the first plasmid phm30contains an incomplete hisi gene, the last gene in thehistidine biosynthesis operon of b. subtilis and part ofthe genes yvca and yvcb of unkown function flankinghisi at the 3´-end. the spectin...

A new system is presented for the generation of recombinant <span style="font-variant: normal; font-style: normal; font-family: Arial-ItalicMT; color: #231f20; font-size: 10pt...

i.p.s. hypergroups are canonical hypergroups such that$[forall(a,x),a+xni x]longrightarrow[a+x=x].$i.p.s. hypergroups were investigated in [1], [2], [3], [4] and it was proved thatif the order is less than 9, they are strongly canonical (see [13]). in this paperwe obtain the sequences of fuzzy sets and of join spaces determined (see [8])by all i.p.s. hypergroups of order seven. for the meaning ...

A potential food-grade cloning vector, pND919, was constructed and transformed into S. thermophilus ST3-1, a plasmid-free strain. The vector contains DNAs from two different food-approved organisms, Streptococcus thermophilus and Lactococcus lactis. The 5.0-kb pND919 is a derivative of the cloning vector pND918 (9.3 kb) and was constructed by deletion of the 4.3-kb region of pND918 which contai...

Calcareous sediments are located in temperate and tropical areas near offshore hydrocarbon industries and petrochemical reserves such as the Persian Gulf of Iran. These sediments have complicated physical properties which make them completely different from silicate soils. Carbonate sediments are mainly formed by the skeletal remains of marine organisms. Therefore, a wide variety of engineering...

Community science-generated biodiversity data can provide essential information for understanding species distributions, behaviors and conservation statuses. However, their utility be limited due to high uncertainty variability in quality, especially small taxonomically difficult organisms like fungi insects. One important set of community-generated that are increasingly used by scientists Rese...