background and objective: although diabetes mellitus is known to be one of the risk factors for dementia but neuropathic changes in the brain of diabetic patients have not been completely revealed. therefore, this research study was done to evaluate structural changes in pyramidal neurons of hippocampal ca1 area of male diabetic rats using golgi-impregnation method. materials and methods: male ...

The Golgi staining technique, also called the black reaction after the stain's color, was developed in the 1870s and 1880s in Italy to make brain cells (neurons) visible under the microscope [3]. Camillo Golgi developed the technique while working with nervous tissue, which required Golgi to examine cell structure under the microscope [3]. Golgi improved upon existing methods of staining, enabl...

A mAb AD7, raised against canine liver Golgi membranes, recognizes a novel, 200-kD protein (p200) which is found in a wide variety of cultured cell lines. Immunofluorescence staining of cultured cells with the AD7 antibody produced intense staining of p200 in the juxtanuclear Golgi complex and more diffuse staining of p200 in the cytoplasm. The p200 protein in the Golgi complex was colocalized ...

BACKGROUND The Golgi-Cox stain is an established method for characterising neuron cell morphology. The method highlights neurite processes of stained cells allowing the complexity of dendritic branching to be measured. NEW METHODS Conventional rapid Golgi and Golgi-Cox methods all require fresh impregnation in unfixed brain blocks. Here, we describe a modified method that gives high quality s...

Golgi staining remains a key method to study neuronal morphology in vivo. Since most protocols delineating modifications of the original staining method lack details on critical steps, establishing this method in a laboratory can be time-consuming and frustrating. Here, we describe the Golgi-Cox staining in such detail that should turn the staining into an easily feasible method for all scienti...

The Golgi-Cox is a gold standard technique to stain the neurons. However the major limitations of the study are that it is time taking and inconsistent. In this paper we describe a modification of the Golgi–Cox method, in which the tissue blocks were immersed in rapid Golgi solution and silver nitrate for staining of neurons in rat brain. The method is simple, reproducible, rapid, inexpensive a...