Background:  Human coagulation factor IX (hFIX) is a glycoprotein with two N-glycosylation sites at the activation peptide. Since the activation peptide is removed in mature hFIX, the exact role of N-glycosylation is unclear. To investigate the role of N-glycosylation in the secretion and activity of hFIX, we inhibited N-glycosylation by tunicamycin in the stable Human Embryonic Kidney (HEK)- c...

introduction: non-enzymatic glycosylation of proteins which is the major cause of diabetic complications, cardiovascular diseases retinopathy, nephropathy and neuropathy can effectively be inhibted by antioxidants. materials and method: the antioxidant effects of several flavonoids, such as rutin, kaempferol, quercetin, apigenin, naringin, morin and biochanin a were studied  on glycosylation of...

abstract introduction: diabetes is one of the most common endocrine diseases spreading rapidly in the world. diabetes complications are classified into acute and chronic. non-enzymatic glycosylation of body proteins such as hemoglobin and albumin is the main cause of pathogenesis in chronic complications of diabetes. protein glycosylation is an oxidative reaction. antioxidants such as vitamin c...

non-enzymatic glycosylation of proteins is one of the key mechanisms in the pathogenesis of diabetic complications. glycation of igg is of special interest due to its possible influence on the functionality of immunoglobulins and overall immuno-competence. the aim of this study was to clarify more details of in vitro glycation of igg and to study the effect of this modification on its interatio...

Alpha-1-proteinase inhibitor (API) is one of the acute phase proteins. Following an inflammatory stimuli the concentration of API increased up to four folds. Accompanying these quantitative changes, there is qualitative alterations in the structure of carbohydrate moiety (glycosylation). To determine the alterations in the glycosylation of API in inflammation, API was isolated from the sera of...

Glycosylation is a conserved set of post-translational modifications that exists in all eukaryotic cells. During the last decade, role glycosylation plant pathogenic fungi has received significant attention and considerable progress been made especially Ustilago maydis Magnaporthe oryzae. Here, we review recent advances our understanding N-glycosylation, O-glycosylation glycosylphosphatidylinos...

Instead of focusing on maximizing glycosylation yield, future syntheses nucleosides via N-glycosylation should aim to use shorter routes, fewer protecting groups and less chromatography achieve more efficient sustainable processes, as this E-factor analysis reveals.

background the mutations of lhbs in pre-s, especially in pre-s2, are definitive in hepatocellular carcinoma (hcc) associated with hbv. however, the mechanisms of the n-glycosylation modification in lhbs are unclear. the n-glycosylation modification of lhbs affects endoplasmic reticulum stress, cell proliferation and its secretion which was further studied. objectives the objectives of our studi...

Abstract Proteins are important ingredients in the food industry for their excellent nutritional, functional and biological properties, while application is limited by sensitivity to environmental stresses such as high temperature, pH changes ionic strength. The Maillard reaction transglutaminase-catalyzed glycosylation two main effective ways improve stability properties of proteins mainly att...