background: monoclonal antibodies (mabs) are essential tools for many molecular im-munology investigations, epitope mapping and molecular modelling, clinical laboratory di-agnostic tests and immunotherapy. humoral immune response of immunized animals largely depends on the nature of antigen and the immunization technique. polysaccharides and heavily-glycosylated proteins are very elusive target...

Infectious bursal disease (IBD), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (IBDV). To improve the immunogenicity of recombinant IBDV subunitvaccine, an attempt was made to find a new way to prepare IBD vaccine containing glycosylated mVP2antigen. Firstly, IBDV mVP2 gene (with a nucleic acid sequence encoding B cell epitope of IBDV(...

infectious bursal disease (ibd), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (ibdv). to improve the immunogenicity of recombinant ibdv subunitvaccine, an attempt was made to find a new way to prepare ibd vaccine containing glycosylated mvp2antigen. firstly, ibdv mvp2 gene (with a nucleic acid sequence encoding b cell epitope of ibdv(...

The CH2 domain is a critical element of the human Immunoglobulin G (IgG) constant region. Although least stable in IgG, it also promising scaffold candidate for developing novel therapeutic approaches. Recently, we succeeded preparing glycosylated and non-glycosylated host organism Pichia pastoris. Herein, verified that glycosylation decreased both, its tendency to aggregate immunogenicity mice...

Two New York (NY) strains of the West Nile (WN) virus were plaque-purified and four variants that had different amino acid sequences at the N-linked glycosylation site in the envelope (E) protein sequence were isolated. The E protein was glycosylated in only two of these strain variants. To determine the relationship between E protein glycosylation and pathogenicity of the WN virus, 6-week-old ...

This study aimed to investigate the effects of galacto-oligogalactose (GOS) glycosylation on structural and functional properties fish gelatin (FG). Results showed that with increase time, grafting degree browning increased, new protein bands increased molecular weight were observed by SDS-PAGE. Structural analysis reduced intrinsic fluorescence intensity surface hydrophobicity FG. FTIR α-helix...