An enzyme-free amperometric sensor based on a heptadecapeptide possessing an electroactive ferrocene (Fc) linker as ferrocene-Gly-Gly-Gly-Gly-Phe-Gly-His-Ile-His-Glu-Gly-Tyr-Gly-Gly-Gly-Gly-Lys-(CH2)4-dithiocyclopentane self-assembled gold substrate was designed and fabricated for specific determination of L-arginine (L-Arg). The detection mechanism is conformational change surface-immobilized ...

Display of peptides on the surface of bacteria offers many new and exciting applications in biotechnology. Fimbriae is a good candidate for epitope display on the surface of bacteria. The potential of CS3 fimbriae of enterotoxigenic E. coli as a display system has been investigated. A novel cell surface display system with metal binding property was developed by using CS3 fimbriae. Short metal ...

Ligand-immobilization to stents and vascular grafts is expected promote endothelialization by capturing flowing endothelial progenitor cells (EPCs). However, the optimized ligand density linker structure have not been fully elucidated. Here, we report that EPCs were selectively captured REDV peptide conjugated with a short linker. The microchannel surface was modified via Gly-Gly-Gly (G3), (Gly...

conclusions the present findings showed that the ta7/7 promoter of ugt1a1 gene accounted for a considerable number of gilbert’s syndrome cases (11.3%). the studied variations had a significant effect on creatine phosphokinase and serum total bilirubin levels. results about 78.9% of the studied subjects had normal homozygous genotypes, and 21.1% were heterozygous for the gly71arg variation. in t...

Glycolate oxidase is a peroxisomal flavoprotein catalyzing the oxidation of glycolate to glyoxylate and plays crucial metabolic roles in green algae, plants, animals. It could serve as biocatalyst for enzymatic production glyoxylate, fine chemical with wide variety applications perfumery, flavor, pharmaceutical agrochemical industries. However, low catalytic activity native levels active enzyme...