نتایج جستجو برای: gluthathione s

تعداد نتایج: 711003  

Journal: :Biotechnology in Animal Husbandry 2005

Journal: :BioTechniques 2010
Hu Tao Wenjun Liu Brandi N Simmons Helen K Harris Timothy C Cox Michael A Massiah

We describe a rapid, simple, and efficient method for recovering glutathione S-transferase (GST)- and His6-tagged maltose binding protein (MBP) fusion proteins from inclusion bodies. Incubation of inclusion bodies with 10% sarkosyl effectively solubilized >95% of proteins, while high-yield recovery of sarkosyl-solubilized fusion proteins was obtained with a specific ratio of Triton X-100 and CH...

Journal: :محیط زیست طبیعی 0
مهرنوش شیرانی کارشناس ارشد بوم‏ شناسی آبزیان. گروه شیلات. دانشکده منابع طبیعی. دانشگاه تهران. کرج. ایران. علیرضا میرواقفی دانشیار گروه شیلات. دانشکده منابع طبیعی. دانشگاه تهران. کرج. ایران. حمید فرحمند دانشیار گروه شیلات. دانشکده منابع طبیعی. دانشگاه تهران. کرج. ایران. محمد عبداللهی استاد مرکز تحقیقات علوم دارویی. گروه سم‏شناسی و داروشناسی دانشکدة داروسازی. دانشگاه علوم پزشکی و خدمات بهداشتی درمانی تهران. تهران. ایران

considerable amounts of different pollutants, especially crude oils, are present in the persian gulf and its northern region, so biomonitoring the effect of these pollutants on its biota is necessary. ethoxyresorufin o-deethylase (erod), gluthathione s-transferase (gst) and catalase (cat) activity were measured from biochemical level in the gills of mudskipper periophthalmus waltoni collected f...

Journal: :Journal of lipid research 1984
Y Sugiyama A Stolz M Sugimoto N Kaplowitz

Binding of lithocholic acid, bilirubin, and gossypol to glutathione S-transferase B (ligandin or transferase YaYc) was compared using four methods. Tryptophan quenching revealed a single high affinity site for bilirubin and gossypol but could not be used for lithocholic acid. Both displacement of the fluorescent probe, 1-anilino-8-naphthalenesulfonate, and spectral changes induced by bilirubin ...

Journal: :Journal of experimental botany 2006
Juan B Barroso Francisco J Corpas Alfonso Carreras María Rodríguez-Serrano Francisco J Esteban Ana Fernández-Ocaña Mounira Chaki María C Romero-Puertas Raquel Valderrama Luisa M Sandalio Luis A del Río

S-nitrosoglutathione (GSNO) is considered a natural nitric oxide (NO.) reservoir and a reactive nitrogen intermediate in animal cells, but little is known about this molecule and its metabolism in plant systems. In this work, using pea plants as a model system, the presence of GSNO in collenchyma cells was demonstrated by an immunohistochemical method. When pea plants were grown with a toxic Cd...

2013
Terri Gelbart

Glutathione synthetase (GSH-S) is one of the two known hereditary causes of glutathione deficiency. We describe a family whose two children have hemolytic anemia. The children's erythrocytes lack GSH and are severely deficient in GSH-S activity. No neurologic findings or 5-oxoprolinuria were present. A concurrent deficiency of glutathione-S-transferase (GST) was also detected in the erythrocyte...

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